PXD027444

PXD027444 is an original dataset announced via ProteomeXchange.

Title	Changes in Mycobacterium avium subsp. paratuberculosis proteome after cultivation in milk
Description	Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne’s disease in cattle. MAP can be either shed directly into milk by infected cows, or introduced via fecal contamination. Viable MAP are detectable in milk and other dairy products, indicating survival of MAP after the pasteurization process. Although direct evidence is still lacking, MAP are discussed as a possible factor in the morbidity for chronic inflammatory bowel diseases in humans, such as Crohn’s disease and ulcerative colitis. Therefore, it is broadly accepted in the scientific community that exposure to MAP, especially through contaminated milk and dairy products, should be kept to a minimum. To gain deeper insight into the role of milk in MAP transmission and the question of why MAP can survive pasteurization, we investigated MAP proteome changes after incubation in milk at 37°C (simulating the environment in the mammary gland) and 4°C (simulating tank milk) as well as incubation in liquid control medium at 37°C.
HostingRepository	PRIDE
AnnounceDate	2021-08-26
AnnouncementXML	Submission_2021-08-26_04:12:41.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roxane Degroote
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Mycobacterium avium subsp. paratuberculosis; NCBI TaxID: 1770;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-20 15:13:03	ID requested
⏵ 1	2021-08-26 04:12:42	announced

Dataset with its publication pending

submitter keyword: Mycobacterium avium subspecies paratuberculosis, dairy product, milk, food safety, LC-MS/MS

Stefanie M Hauck
contact affiliation	Research Unit Protein Science, Helmholtz Center Munich, German Research Center for Environmental Health GmbH, Germany
contact email	hauck@helmholtz-muenchen.de
lab head
Roxane Degroote
contact affiliation	LMU Munich, Veterinary Sciences, Chair of Physiology, Lena-Christ-Straße 48, 82152 Planegg/Martinsried, Germany
contact email	r.degroote@lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD027444

PRIDE project URI

• PRIDE
  1. PXD027444
     1. Label: PRIDE project
     2. Name: Changes in Mycobacterium avium subsp. paratuberculosis proteome after cultivation in milk