PXD027408 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Viral immune evasions differentially impact cell surface presentation of MHC I allomorphs already at the peptide-loading complex |
Description | The transporter associated with antigen processing (TAP) translocates peptides from the cytosol into the endoplasmic reticulum (ER) where they are loaded onto major histocompatibility class I (MHC I) molecules. Stable peptide-MHC I complexes travel to the cell surface and present their antigenic cargo to cytotoxic T lymphocytes. As central part of the peptide-loading complex (PLC), TAP is targeted by several viral proteins, which inhibit peptide translocation and thereby impact MHC I-mediated immune responses. However, it is still poorly understood whether the MHC I allotypes are differently affected by TAP inhibition. Here, we show that conditional expression of herpes simplex virus (HSV) ICP47 suppresses surface presentation of the MHC I allotypes HLA-A and HLA-C, but not of HLA-B, while expression of cytomegalovirus (CMV) US6 reduces surface levels of all allotypes. This difference is directly reflected in a specific enrichment of HLA-B molecules at US6 arrested PLC. Since ICP47 and US6 inhibit TAP in different transport-incompetent conformations, our data imply that MHC I allomorphs are preferentially recruited or trapped at the PLC leading to selective downregulation of MHC I surface presentation. Our findings suggest that viral immune evasions not only inhibit peptide supply to the ER by TAP, but also modulate the assembly and chaperone activity of the PLC. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_05:29:53.914.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marie Barth |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-19 02:37:32 | ID requested | |
⏵ 1 | 2022-02-17 05:29:54 | announced | |
Publication List
Sethumadhavan S, Barth M, Spaapen RM, Schmidt C, Trowitzsch S, Tamp, é R, I at the peptide-loading complex. Sci Rep, 12(1):1516(2022) [pubmed] |
Keyword List
submitter keyword: mass spectrometry, ER quality control, MHC I biogenesis, major histocompatibility complex class I, peptide-loading complex, viral immune evasion |
Contact List
Carla Schmidt |
contact affiliation | Interdisciplinary research center HALOmem, Institute of Biochemistry and Biotechnology, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, Halle, Germany |
contact email | carla.schmidt@biochemtech.uni-halle.de |
lab head | |
Marie Barth |
contact affiliation | HALOmem, Martin Luther University Halle-Wittenberg |
contact email | marie.barth@bct.uni-halle.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027408 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027408
- Label: PRIDE project
- Name: Viral immune evasions differentially impact cell surface presentation of MHC I allomorphs already at the peptide-loading complex