PXD027407

PXD027407 is an original dataset announced via ProteomeXchange.

Title	Rat cell supernatant and the separation of supernatant LC-MSMS
Description	We find one organ of body is special useful for skeletal muscle recovery from indury. To identify the active substance in TCM(special organ cell culturing supernatant which can activate satellite cell proliferation), we prepared serum-free TCM and also separate several fractions, subjected them to LC-MS/MS analysis. To our surprise, most proteins extracted belong to metabolite interconversion enzymes, nucleic acid binding proteins, cytoskeletal proteins, protein modifying enzymes, membrane traffic proteins and so on, but no cytokine was detected.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:27:02.941.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhang xuena
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	dehydrated residue; monohydroxylated residue; formylated residue; deamidated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-19 02:37:27	ID requested
⏵ 1	2022-02-17 05:27:03	announced

Zheng YY, Wang Y, Chen X, Wei LS, Wang H, Tao T, Zhou YW, Jiang ZH, Qiu TT, Sun ZY, Sun J, Wang P, Zhao W, Li YQ, Chen HQ, Zhu MS, Zhang XN, The thymus regulates skeletal muscle regeneration by directly promoting satellite cell expansion. J Biol Chem, 298(1):101516(2022) [pubmed]

submitter keyword: rat,cell supernatant, LC-MSMS

Zhang Xuena
contact affiliation	State Key Laboratory of Pharmaceutical Biotechnology, Model Animal Research Center of Medical School and Gulou Hospital affiliated Medical School, Nanjing University
contact email	zhangxn@nju.edu.cn
lab head
zhang xuena
contact affiliation	Nanjing University
contact email	zhangxn@nju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027407

PRIDE project URI

• PRIDE
  1. PXD027407
     1. Label: PRIDE project
     2. Name: Rat cell supernatant and the separation of supernatant LC-MSMS