PXD027403

PXD027403 is an original dataset announced via ProteomeXchange.

Title	Structural mapping of the surfaces of fibrillar 1N3R and 1N4R human tau
Description	The structural characterization of pathogenic tau filaments that accumulate in tauopathies, including Alzheimer disease, is key for understanding their pathogenic function and identify the structural determinants of distinct tau filaments involved in distinct tauopathies. Fibrillar tau surfaces play a crucial role in tau fibrils interaction with various protein partners and binding to neurons, a key step for their prion-like propagation propensity. In order to identify the amino acid residues stretches that are exposed and those that are not exposed to the solvent within tau fibrils, we used structural proteomic approaches: proteolysis and molecular covalent surface painting using NHS-Biotin followed by MS-based analysis of the proteolytic products. We compared the solvent accessible amino acid residues on the surface of htau proteins upon assembly of 1N3R and 1N4R human htau monomers into fibrils in vitro. We mapped the amino acid segments exposed or unexposed on the surface of htau fibrils by determining the accessibility GluC enzyme and NHS-biotin followed by proteolysis. The proteolytic peptides were analyzed by nanoLC-MSMS and processed using Mascot for their identification and PeakView for intensity extraction of labeled and unlabeled proteolytic peptides.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:26:21.011.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Virginie Redeker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 4600

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-19 02:36:52	ID requested
⏵ 1	2022-02-17 05:26:21	announced

Caroux E, Redeker V, Madiona K, Melki R, Structural mapping techniques distinguish the surfaces of fibrillar 1N3R and 1N4R human tau. J Biol Chem, 297(5):101252(2021) [pubmed]

submitter keyword: Structural proteomics

proteolysis

chemical surface modification

NHS-Biotin

nanoLC-MSMS, tau fibrils

Virginie REDEKER
contact affiliation	MIRCen, CNRS UMR 9199, Fontenay-aux-Roses, France
contact email	virginie.redeker@cnrs.fr
lab head
Virginie Redeker
contact affiliation	CNRS
contact email	virginie.redeker@cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027403

PRIDE project URI

• PRIDE
  1. PXD027403
     1. Label: PRIDE project
     2. Name: Structural mapping of the surfaces of fibrillar 1N3R and 1N4R human tau