PXD027402

PXD027402 is an original dataset announced via ProteomeXchange.

Title	Evaluation of burbot Lota lota spermatozoon motility, lipid composition, protein profile, and swelling rate following change of temperature and osmolality
Description	Despite available information on the roles of osmotic pressure, potassium (K+), and calcium (Ca2+) in activation of Eurasian burbot spermatozoon motility, the changes in sperm biochemical composition and mechanisms underlying their activation at temperatures above optimal spawning temperature is still unclear. We explored sperm swelling, lipid composition, and proteome in relation to osmolality and temperature of swimming medium. Higher temperatures increase burbot spermatozoa vulnerability to osmotic pressure, decreasing motility in extremely hypotonic media, and the presence of Ca2+ decreases swelling of spermatozoa. A role of swelling in activation of spermatozoa motility at high temperature was rejected. We observed no differences in protein profile or lipid composition with respect to activation of burbot spermatozoa at high temperature. Burbot sperm activation occurs at higher temperatures without modification of the spermatozoon membrane. Elucidation of the intrinsic signaling pathway of burbot spermatozoon spontaneous activation requires further study.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:06:26.832.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dmitry Loginov
SpeciesList	scientific name: Lota lota; NCBI TaxID: 69944;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-19 02:36:52	ID requested
1	2022-09-20 06:02:59	announced
⏵ 2	2023-11-14 07:06:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Biomolecule, Protein, Fish Lipid, Sperm activation, Osmolality

Jan Sterba
contact affiliation	Faculty of Science, University of South Bohemia
contact email	sterbaj@prf.jcu.cz
lab head
Dmitry Loginov
contact affiliation	BioCeV - Institute of Microbiology of the CAS
contact email	dloginov@jcu.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027402
     1. Label: PRIDE project
     2. Name: Evaluation of burbot Lota lota spermatozoon motility, lipid composition, protein profile, and swelling rate following change of temperature and osmolality