PXD027396
PXD027396 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Papi interacting proteins in mitochondrial fraction of BmN4 cells |
Description | We incubated Papi-Flag in BmN4 mitochondrial lysates in the presence of 32P-ATP. Upon incubation, Papi-Flag was 32P-labeled, suggesting the presence of kinases for Papi in the fraction. Papi-Flag was immunoprecipitated after incubation in mitochondrial lysates under ATP-free conditions. The proteins were eluted from the beads with a high salt buffer and subjected to LC-MS/MS. This identified six proteins of which were annotated as kinases. |
HostingRepository | jPOST |
AnnounceDate | 2022-02-01 |
AnnouncementXML | Submission_2022-09-18_02:28:45.389.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lumi Negishi |
SpeciesList | scientific name: Bombyx mori; NCBI TaxID: 7091; |
ModificationList | S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-07-19 00:28:31 | ID requested | |
1 | 2022-01-31 17:56:26 | announced | |
2 | 2022-04-14 22:26:31 | announced | 2022-04-15: Updated PubMed. |
⏵ 3 | 2022-09-18 02:28:46 | announced | 2022-09-18: Updated FTP location. |
Publication List
Yamada H, Nishida KM, Iwasaki YW, Isota Y, Negishi L, Siomi MC, Siwi cooperates with Par-1 kinase to resolve the autoinhibitory effect of Papi for Siwi-piRISC biogenesis. Nat Commun, 13(1):1518(2022) [pubmed] |
Keyword List
submitter keyword: Papi, BmN4, |
Contact List
Mikiko C. Siomi | |
---|---|
lab head | |
Lumi Negishi | |
contact affiliation | The University of Tokyo |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001245/ |