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PXD027396

PXD027396 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePapi interacting proteins in mitochondrial fraction of BmN4 cells
DescriptionWe incubated Papi-Flag in BmN4 mitochondrial lysates in the presence of 32P-ATP. Upon incubation, Papi-Flag was 32P-labeled, suggesting the presence of kinases for Papi in the fraction. Papi-Flag was immunoprecipitated after incubation in mitochondrial lysates under ATP-free conditions. The proteins were eluted from the beads with a high salt buffer and subjected to LC-MS/MS. This identified six proteins of which were annotated as kinases.
HostingRepositoryjPOST
AnnounceDate2022-02-01
AnnouncementXMLSubmission_2022-09-18_02:28:45.389.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLumi Negishi
SpeciesList scientific name: Bombyx mori; NCBI TaxID: 7091;
ModificationListS-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-19 00:28:31ID requested
12022-01-31 17:56:26announced
22022-04-14 22:26:31announced2022-04-15: Updated PubMed.
32022-09-18 02:28:46announced2022-09-18: Updated FTP location.
Publication List
Yamada H, Nishida KM, Iwasaki YW, Isota Y, Negishi L, Siomi MC, Siwi cooperates with Par-1 kinase to resolve the autoinhibitory effect of Papi for Siwi-piRISC biogenesis. Nat Commun, 13(1):1518(2022) [pubmed]
Keyword List
submitter keyword: Papi, BmN4,
Contact List
Mikiko C. Siomi
lab head
Lumi Negishi
contact affiliationThe University of Tokyo
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001245/