PXD027395

PXD027395 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Phosphorylation sites that influences the RNA-binding activity of Papi
Description	We conducted UV cross-linking immunoprecipitation (CLIP) with and without phosphatase inhibitor and compared the outcome. The topmost band on the western blots appeared more prominent with the inhibitor. The RNA-binding activity of the band was also stronger with the inhibitor. We isolated the band and subjected to LC-MS/MS to identify the phosphorylated residues.
HostingRepository	jPOST
AnnounceDate	2022-02-01
AnnouncementXML	Submission_2022-09-18_03:52:19.941.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lumi Negishi
SpeciesList	scientific name: Bombyx mori; NCBI TaxID: 7091;
ModificationList	S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-19 00:26:53	ID requested
1	2022-01-31 17:57:51	announced
2	2022-05-17 22:59:49	announced	2022-05-18: Updated PubMed.
⏵ 3	2022-09-18 03:52:20	announced	2022-09-18: Updated FTP location.

Publication List

Yamada H, Nishida KM, Iwasaki YW, Isota Y, Negishi L, Siomi MC, Siwi cooperates with Par-1 kinase to resolve the autoinhibitory effect of Papi for Siwi-piRISC biogenesis. Nat Commun, 13(1):1518(2022) [pubmed]

Yamada H, Nishida KM, Iwasaki YW, Isota Y, Negishi L, Siomi MC, Siwi cooperates with Par-1 kinase to resolve the autoinhibitory effect of Papi for Siwi-piRISC biogenesis. Nat Commun, 13(1):1518(2022) [pubmed]

Keyword List

submitter keyword: Papi, Phosphorylation

submitter keyword: Papi, Phosphorylation

Contact List

Mikiko C. Siomi
lab head
Lumi Negishi
contact affiliation	The University of Tokyo
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001244/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001244/