PXD027391

PXD027391 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the thylakoid membrane in Arabidopsis in the lab versus the field
Description	The plant chloroplast thylakoid membrane must respond to environmental variations in light intensity to maximise the efficiency of photosynthesis and minimise photo-oxidative stress. Plants respond to changing light intensity in the short-term (seconds to minutes) through altered regulation of the structure and function of existing thylakoid components, whereas long-term acclimation (hours to days) involves changes in gene expression, protein synthesis and degradation, which modulate the composition of the thylakoid membrane. Here we have investigated the long-term changes in the thylakoid membrane composition in Arabidopsis thaliana plants acclimated to a controlled laboratory environment to those acclimated to the field using quantitative label-free proteomics in combination with biochemical and structural analyses.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:31:40.831.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Flannery
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-18 23:50:43	ID requested
⏵ 1	2022-02-17 05:31:41	announced

Flannery SE, Pastorelli F, Wood WHJ, Hunter CN, Dickman MJ, Jackson PJ, Johnson MP, grown in laboratory and field conditions. Plant Direct, 5(10):e355(2021) [pubmed]

submitter keyword: LC-MSMS, thylakoid, Arabidopsis, Q Exactive HF

Matthew Johnson
contact affiliation	Department of Molecular Biology and Biotechnology, University of Sheffield
contact email	matt.johnson@sheffield.ac.uk
lab head
Sarah Flannery
contact affiliation	University of Sheffield
contact email	sflannery1@sheffield.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027391

PRIDE project URI

• PRIDE
  1. PXD027391
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the thylakoid membrane in Arabidopsis in the lab versus the field