PXD027378
PXD027378 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Analysis of phosphorylation sites of ZmRR1 phosphorylated by ZmMAPK8 |
| Description | To identify the phosphorylation site of ZmRR1 by ZmMPK8 in vitro, 10 µg GST-ZmRR1 and 1 µg His-ZmMPK8 purified proteins were incubated in 20 µl of protein kinase reaction buffer containing 20 mM MgCl2, 50 mM Tris-HCl pH 7.5, 1 mM DTT, and 50 µM ATP, at 30°C for 30 min. The reaction products were reduced by DTT and alkylated by IAM (Iodoacetamide), followed by digestion with trypsin (pH 8.5) at 37°C for 12 h. The results were analyzed by LC-MS/MS |
| HostingRepository | iProX |
| AnnounceDate | 2021-07-16 |
| AnnouncementXML | Submission_2022-12-19_18:08:17.789.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rong Zeng |
| SpeciesList | scientific name: Zea mays; NCBI TaxID: 4577; |
| ModificationList | phosphorylated residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-07-18 20:48:10 | ID requested | |
| 1 | 2021-07-18 20:48:38 | announced | |
| ⏵ 2 | 2022-12-19 18:08:18 | announced | 2022-12-20: Update information publication. |
Publication List
| Zeng R, Li Z, Shi Y, Fu D, Yin P, Cheng J, Jiang C, Yang S, Natural variation in a type-A response regulator confers maize chilling tolerance. Nat Commun, 12(1):4713(2021) [pubmed] |
Keyword List
| submitter keyword: ZmRR1,ZmMAPK8,phospho-sites |
Contact List
| Shuhua Yang | |
|---|---|
| contact affiliation | China Agricultural University |
| contact email | yangshuhua@cau.edu.cn |
| lab head | |
| Rong Zeng | |
| contact affiliation | China Agricultural University |
| contact email | 1603998769@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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