PXD027352

PXD027352 is an original dataset announced via ProteomeXchange.

Title	Sub-centrosomal mapping identifies augmin-TuRC as part of a centriole-stabilizing scaffold
Description	Centriole biogenesis and maintenance are crucial for cells to generate cilia and assemble centrosomes that function as microtubule organizing centers (MTOCs). Centriole biogenesis and MTOC function both require the microtubule nucleator -tubulin ring complex (TuRC). It is widely accepted that TuRC nucleates microtubules from the pericentriolar material (PCM) that is associated with the proximal part of centrioles. However, TuRC also localizes more distally and in the centriole lumen, but the significance of these findings is unclear. Here we identify spatially and functionally distinct sub-populations of centrosomal TuRC. Luminal localization is mediated by augmin, which is linked to the centriole inner scaffold through POC5. Disruption of luminal localization impairs centriole integrity and interferes with cilium assembly. Defective ciliogenesis is also observed in TuRC mutant fibroblasts from a patient suffering from microcephaly with chorioretinopathy. These results identify a novel, non-canonical role of augmin-γTuRC in the centriole lumen that is linked to human disease.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:25:10.728.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marina Gay
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-15 23:44:14	ID requested
⏵ 1	2022-02-17 05:25:11	announced

Schweizer N, Haren L, Dutto I, Viais R, Lacasa C, Merdes A, L, ü, ders J, TuRC as part of a centriole-stabilizing scaffold. Nat Commun, 12(1):6042(2021) [pubmed]

submitter keyword: centriole, augmin, TuRC, microtubules, ciliogenesis

Jens Lüders
contact affiliation	Microtubule Organization Group Mechanisms of Disease Programme Institute for Research in Biomedicine (IRB Barcelona) The Barcelona Institute of Science and Technology (BIST)
contact email	jens.luders@irbbarcelona.org
lab head
Marina Gay
contact affiliation	Institute for Research in Biomedicine
contact email	marina.gay@irbbarcelona.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027352

PRIDE project URI

• PRIDE
  1. PXD027352
     1. Label: PRIDE project
     2. Name: Sub-centrosomal mapping identifies augmin-TuRC as part of a centriole-stabilizing scaffold