PXD027346

PXD027346 is an original dataset announced via ProteomeXchange.

Title	The trypanosome Rhomboid protease RHBDL4 promotes retrotranslocation of aggregation-prone proteins for degradation
Description	: In the endoplasmic reticulum (ER), the ER-associated degradation (ERAD) pathway targets incorrectly folded and unassembled proteins into the cytoplasm for turnover by the proteasome. Previously, we showed that the ER-resident rhomboid protease RHBDL4, together with p97, mediates membrane protein degradation. However, whether RHBDL4 acts in concert with additional ERAD components is unclear, and its full substrate spectrum remains to be defined. SILAC-based shotgun proteomics and immunoprecipitation experiments revealed that endogenously-tagged RHBDL4 interacts with the ERAD erlin complex. The interaction was validated with immunoprecipitation experiments of endogenous RHBDL4, Erlin1 and Erlin2 to show robust co-purification of the respective interaction partner.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:41:37.049.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Ruppert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-15 09:30:32	ID requested
1	2022-10-14 04:33:42	announced
⏵ 2	2023-11-14 07:41:40	announced	2023-11-14: Updated project metadata.

Bock J, K, ü, hnle N, Knopf JD, Landscheidt N, Lee JG, Ye Y, Lemberg MK, Rhomboid protease RHBDL4 promotes retrotranslocation of aggregation-prone proteins for degradation. Cell Rep, 40(6):111175(2022) [pubmed]

submitter keyword: intramembrane proteolysis / rhomboid family protein / ER-associated protein degradation / prohibitin family proteins Erlin1 and Erlin2 / protein aggregates

Thomas Ruppert
contact affiliation	Zentrum für Molekulare Biologie (ZMBH), Mass Spectrometry and Proteomics
contact email	t.ruppert@zmbh.uni-heidelberg.de
lab head
Thomas Ruppert
contact affiliation	ZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg
contact email	t.ruppert@zmbh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD027346

PRIDE project URI

• PRIDE
  1. PXD027346
     1. Label: PRIDE project
     2. Name: The trypanosome Rhomboid protease RHBDL4 promotes retrotranslocation of aggregation-prone proteins for degradation