PXD027344

PXD027344 is an original dataset announced via ProteomeXchange.

Title	Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines
Description	Vector-based SARS-CoV-2 vaccines such as AstraZeneca's ChAdOx1 nCoV-19 have been associated with vaccine-induced thrombosis (TTS/VITT). We studied ChAdOx1 nCov-19 (AstraZeneca) and Ad26.COV2.S (Johnson & Johnson) and compared the vaccines using proteomics with mass spectrometric measurements. We could demonstrate that ChAdOx1 nCoV-19 contained significant amounts of human proteins in addition to adenoviral proteins. This was not the case for Ad26.COV2.S. Among the detected human proteins, proteasome subunits beta 1/delta and beta 5/MB1 were identified. In summary, both studied SARS-CoV-2 vaccines are markedly different concerning their content of contaminating proteins, which is most likely a determining factor for different frequencies of TTS/VITT after their application.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:45:16.407.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Hentschker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-15 09:30:19	ID requested
1	2023-05-04 07:22:42	announced
2	2023-11-14 08:45:29	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:45:17	announced	2024-10-22: Updated project metadata.

10.3324/haematol.2021.280154;

Michalik S, Siegerist F, Palankar R, Franzke K, Schindler M, Reder A, Seifert U, Cammann C, Wesche J, Steil L, Hentschker C, Gesell-Salazar M, Reisinger E, Beer M, Endlich N, Greinacher A, V, ö, lker U, Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines. Haematologica, 107(4):947-957(2022) [pubmed]

ProteomeXchange project tag: Covid-19

submitter keyword: Ad26.COV2.S, SARS-CoV-2, vector vaccines,ChAdOx1

Uwe Völker
contact affiliation	University Medicine Greifswald, Interfaculty Institute of Genetics and Functional Genomics, Department of Functional Genomics
contact email	voelker@uni-greifswald.de
lab head
Christian Hentschker
contact affiliation	University Medicine Greifswald, Interfaculty Institute of Genetics and Functional Genomics, Department of Functional Genomics
contact email	christian.hentschker@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD027344

PRIDE project URI

• PRIDE
  1. PXD027344
     1. Label: PRIDE project
     2. Name: Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines