PXD027337

PXD027337 is an original dataset announced via ProteomeXchange.

Title	Regulatory phosphosite on Mec1 and control of RNAP11 and PIII during replication stress
Description	Yeast sensor checkpoint kinase Mec1 is phosphorylated at Ser1991 upon HU replication stress. The phospho-accepter mutant (mec1-S1991A) confers HU sensitivity and synthetic sickness with the mutants that aggravate replication and transcription collision. To understand how the entire chromatin proteome (chromatome) responds to replication stress, we first investigated the global chromatin-bound proteins in S-phase yeast cells in the presence and absence of HU. Second, we performed phospho-proteome analysis in wild-type and mec1-S1991A mutant to find the S1991-phospho-dependent targets of Mec1 in the presence and absence of HU. Samples were duplicated in chromatome and triplicated in phospho-proteome analysis.
HostingRepository	PRIDE
AnnounceDate	2021-09-28
AnnouncementXML	Submission_2021-09-27_23:58:02.326.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jan Seebacher
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-15 09:29:39	ID requested
⏵ 1	2021-09-27 23:58:02	announced

Dataset with its publication pending

submitter keyword: Yeast, Mec1, replication stress, chromatome, phosphoproteomics, LC-MSMS

Susan Gasser
contact affiliation	Friedrich Miescher Institute for Biomedical Research (FMI) Basel, Switzerland
contact email	susan.gasser@fmi.ch
lab head
Jan Seebacher
contact affiliation	FMI Basel
contact email	jan.seebacher@fmi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD027337

PRIDE project URI

• PRIDE
  1. PXD027337
     1. Label: PRIDE project
     2. Name: Regulatory phosphosite on Mec1 and control of RNAP11 and PIII during replication stress