PXD027335

PXD027335 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of glioblastoma stem cells, human astrocyte, and T98G glioblastoma cell line
Description	With HiRIEF LC-MS/MS shotgun proteomics, we analysed 6 patient-derived glioblastoma stem cells (BT stem cells) and compared them to an astrocyte line (CliniSciences, Guidonia Montecelio, Italy) and a more differentiated glioblastoma cell line (T98G). Each of the 8 cell line sample was run in triplicates in a total of three TMT10 sets, assigning each replicate in a separate TMT10 set, using 2 internal standards per set. The three TMT10 sets were ran in two experiments, first on immobilized pH gradient (IPG) 3-10 isoelectric focusing (IEF) strips, and then on IPG 3.7-4.9 IEF strips.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:31:37.248.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haris Babačić
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-15 09:29:27	ID requested
1	2022-12-23 10:38:50	announced
⏵ 2	2023-11-14 07:31:40	announced	2023-11-14: Updated project metadata.

Baba, č, i, ć H, Galardi S, Umer HM, Hellstr, ö, m M, Uhrbom L, Maturi N, Cardinali D, Pellegatta S, Michienzi A, Trevisi G, Mangiola A, Lehti, ö J, Ciafr, è SA, Pernemalm M, Glioblastoma stem cells express non-canonical proteins and exclusive mesenchymal-like or non-mesenchymal-like protein signatures. Mol Oncol, 17(2):238-260(2023) [pubmed]

submitter keyword: glioblastoma, stem cells, HiRIEF, brain cancer, T98G, cell line, LC-MS/MS, astrocyte

Janne Lehtiö
contact affiliation	Department of Oncology and Pathology, Science for Life Laboratory, Karolinska Institute
contact email	janne.lehtio@ki.se
lab head
Haris Babačić
contact affiliation	1. Department of Oncology-Pathology, Karolinska Institute, Stockholm; 2. Proteogenomics Facility, Science for Life Laboratory
contact email	haris.babacic@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD027335

PRIDE project URI

• PRIDE
  1. PXD027335
     1. Label: PRIDE project
     2. Name: Proteomic profiling of glioblastoma stem cells, human astrocyte, and T98G glioblastoma cell line