PXD027330

PXD027330 is an original dataset announced via ProteomeXchange.

Title	Deubiquitinating enzymes and the proteasome regulate unique sets of ubiquitin substrates.
Description	The ubiquitin-proteasome axis has been extensively explored at a system-wide level, but the impact of deubiquitinating enzymes (DUBs) on the ubiquitinome remains largely unknown. Using UbiSite technology and inhibitors, we have compared the contributions of the proteasome and DUBs on the ubiquitinome. We uncovered large differential dynamic Ub signalling networks with substrates and sites uniquely regulated by DUBs or by the proteasome, highlighting the role of DUBs in degradation-independent ubiquitin signalling. DUBs regulate substrates via nearly 40,000 unique sites. Moreover, we found that ubiquitin conjugated to SUMO2/3 forms a unidirectional proteasomal degradation signal with strikingly rapid kinetics compared to ubiquitin polymers only. We found that PARP1 is hyper ubiquitinated in response to DUB inhibition, increasing its enzymatic activity. Our findings highlight the key regulatory roles of DUBs on ubiquitin dynamics.
HostingRepository	PRIDE
AnnounceDate	2022-05-23
AnnouncementXML	Submission_2022-05-23_04:50:11.514.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fredrik Trulsson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-15 09:28:57	ID requested
⏵ 1	2022-05-23 04:50:12	announced

Trulsson F, Akimov V, Robu M, van Overbeek N, Berrocal DAP, Shah RG, Cox J, Shah GM, Blagoev B, Vertegaal ACO, Deubiquitinating enzymes and the proteasome regulate preferential sets of ubiquitin substrates. Nat Commun, 13(1):2736(2022) [pubmed]

submitter keyword: Human, U2OS, His10-Ub, Ubiquitin, TAK243, MG132, PR619

Alfred Vertegaal
contact affiliation	Leiden University Medical Center
contact email	A.C.O.Vertegaal@lumc.nl
lab head
Fredrik Trulsson
contact affiliation	LUMC
contact email	c.f.b.trulsson@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD027330

PRIDE project URI

• PRIDE
  1. PXD027330
     1. Label: PRIDE project
     2. Name: Deubiquitinating enzymes and the proteasome regulate unique sets of ubiquitin substrates.