PXD027327

PXD027327 is an original dataset announced via ProteomeXchange.

Title	Cleavage of hemagglutinin from influenza A, at varying pH and over a twenty-four hour time period in the presence of cathepsin B, H and S
Description	Pathogen-derived peptides presented by MHCII that are best able to activate cognate CD4+ T cells and initiate an adaptive immune response are termed immunodominant. The exact mechanisms and molecular determinants of antigen processing that contribute to immunodominance remain unclear. Recently, attention has been called to the events of proteolytic cleavage as a potential determinant. Here, we compare data generated through cleavage of hemagglutinin from influenza A, at varying pH and over a twenty-four hour time period in the presence of cathepsin B, H and S, to an immunodominant hierarchy previously established with an ex-vivo mouse model. We show that cleavage alone does not directly predict immunodominance, however resistance to digestion emerged as a determinant of immunodominance. Moreover, pH conditions contribute to shaping the peptidome available for MHCII binding and the size of the pools of unique peptides associated with immunodominance. These differences suggest the presence of multiple antigen processing pathways through which resistance to proteolytic cleavage and peptide redundancy may result in a more diverse peptidome for presentation.
HostingRepository	PRIDE
AnnounceDate	2021-08-16
AnnouncementXML	Submission_2021-08-16_09:01:16.750.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027327
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Greg Sabat
SpeciesList	scientific name: Influenza A virus (A/New Caledonia/20-JY2/1999(H1N1)); NCBI TaxID: 1346349;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-15 09:28:43	ID requested
⏵ 1	2021-08-16 09:01:17	announced

Dataset with its publication pending

submitter keyword: Cathepsin, MHCII, influenza A, hemagglutinin, immunodominance, LC-MS/MS

Dr. Thomas Kuhn
contact affiliation	Department of Chemistry and Biochemistry, University of Alaska Fairbanks, Fairbanks, AK
contact email	tbkuhn@alaska.edu
lab head
Greg Sabat
contact affiliation	University of Wisconsin
contact email	gsabat@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027327
     1. Label: PRIDE project
     2. Name: Cleavage of hemagglutinin from influenza A, at varying pH and over a twenty-four hour time period in the presence of cathepsin B, H and S