PXD027326

PXD027326 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Brain microvascular endothelial cells: INSR KD
Description	The insulin receptor (INSR) mediates insulin signaling to modulate cellular functions. Although INSR is expressed at the blood-brain barrier (BBB), its role in the modulation of BBB function is poorly understood. Therefore, in this study, we aimed to analyze the effect of INSR knockdown on the expression levels of functional proteins at the BBB. We established the INSR-knockdown cell line (shINSR) using human cerebral microvascular endothelial cells (hCMEC/D3). The cellular proteome was analyzed using quantitative proteomics. Protein expression levels were quantified by targeted proteomics.
HostingRepository	jPOST
AnnounceDate	2021-12-25
AnnouncementXML	Submission_2022-09-18_02:47:46.224.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sumio Ohtsuki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-15 01:04:27	ID requested
1	2021-12-24 07:00:06	announced
⏵ 2	2022-09-18 02:47:47	announced	2022-09-18: Updated FTP location.

Publication List

Nagano H, Ito S, Masuda T, Ohtsuki S, Effect of Insulin Receptor-Knockdown on the Expression Levels of Blood-Brain Barrier Functional Proteins in Human Brain Microvascular Endothelial Cells. Pharm Res, 39(7):1561-1574(2022) [pubmed]

Nagano H, Ito S, Masuda T, Ohtsuki S, Effect of Insulin Receptor-Knockdown on the Expression Levels of Blood-Brain Barrier Functional Proteins in Human Brain Microvascular Endothelial Cells. Pharm Res, 39(7):1561-1574(2022) [pubmed]

Keyword List

submitter keyword: brain barrier, insulin receptor, SWATH

submitter keyword: brain barrier, insulin receptor, SWATH

Contact List

Sumio Ohtsuki
lab head
Sumio Ohtsuki
contact affiliation	Kumamoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001256/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001256/