⮝ Full datasets listing

PXD027314

PXD027314 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleJuvenile hormone membrane signaling phosphorylates Hsp83 and promotes Met nuclear import
DescriptionJH modulates phosphorylation modification of components of its intracellular signaling through putative membrane pathway. As a transcriptional factor, Met phosphorylation is contributed to its transcriptional activity, but whether phosphorylation modification affects Met nuclear import is unknown. Here, we report that phosphorylation sites are dispensable for NLS activity whether JH is present or not. Interestingly, binding affinity of Met and Hsp83 fails to increase by adding JH is resulted from the mutant of JH-regulated phosphorylation of Hsp83 at S219. JH also increase binding affinity of Hsp83 and 14-3-3 proteins independent on S219. 14-3-3 proteins negatively regulated JH activity through sequestrating Met in the cytoplasm that dependent on Hsp83, which acts as a bridge between Met/Gce and 14-3-3 proteins. These results suggest that JH membrane pathway phosphorylates either Met or Hsp83 to promote JH intracellular signaling, meanwhile, a negative feedback loop forms between the 14-3-3 proteins and Hsp83.
HostingRepositoryiProX
AnnounceDate2021-07-15
AnnouncementXMLSubmission_2022-12-19_18:07:26.682.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSuning Liu
SpeciesList scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-14 20:13:28ID requested
12021-07-14 20:14:10announced
22022-12-19 18:07:27announced2022-12-20: Update information publication.
Publication List
Gao Y, Chen N, Zhang X, Li EY, Luo W, Zhang J, Zhang W, Li S, Wang J, Liu S, Juvenile Hormone Membrane Signaling Enhances its Intracellular Signaling Through Phosphorylation of Met and Hsp83. Front Physiol, 13():872889(2022) [pubmed]
Keyword List
submitter keyword: juvenile hormone, Hsp83, Met
Contact List
Sheng Li
contact affiliationSouth China Normal University
contact emaillisheng@scnu.edu.cn
lab head
Suning Liu
contact affiliationSouth China Normal University
contact emailliusuning@m.scnu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI