PXD027310

PXD027310 is an original dataset announced via ProteomeXchange.

Title	Fast Fluoroalkylation of Proteins Uncovers Structure and Dynamics of Biological Macromolecules
Description	Stable covalent labeling of proteins in combination with mass spectrometry has been established as a complementary technique to classical structural methods, such as X Ray, NMR, or Cryo-EM used for protein structure determination. Although the current stable covalent labeling techniques enable to monitor protein solvent accessible areas with sufficient spatial resolution, there is still high demand for alternative, less complicated and inexpensive approaches. Here, we introduce a new covalent labeling method based on Fast Fluoroalkylation of Proteins (FFAP). The FFAP uses fluoroalkyl radicals formed by reductive decomposition of Togni reagents with ascorbic acid for labeling of proteins on a time scale of seconds. The feasibility of FFAP for effective labeling of proteins was demonstrated by monitoring differential amino acids modi-fication of native horse heart apomyoglobin/holomyoglobin and human haptoglobin-hemoglobin complex. The obtained data confirmed the Togni reagent-mediated FFAP as an advantageous alternative method for covalent labeling in applications, such as protein fingerprinting, epitope mapping and surface mapping of proteins (and their complexes) in general.
HostingRepository	PRIDE
AnnounceDate	2022-02-14
AnnouncementXML	Submission_2022-02-14_03:53:49.267.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zdenek Kukacka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; complex glycosylation; iodoacetamide derivatized residue
Instrument	Bruker Daltonics solarix series

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-14 09:26:39	ID requested
1	2021-12-30 14:11:53	announced
⏵ 2	2022-02-14 03:53:50	announced	2022-02-14: Updated project metadata.

Fojt, í, k L, Fiala J, Pompach P, Chmel, í, k J, Matou, š, ek V, Beier P, Kuka, č, ka Z, Nov, á, k P, Fast Fluoroalkylation of Proteins Uncovers the Structure and Dynamics of Biological Macromolecules. J Am Chem Soc, 143(49):20670-20679(2021) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: radical labeling, mass spectrometry, protein footprinting, conformational changes, fluoroalkylation, hyperva-lent iodine reagents,protein structure, Togni reagents, protein fingerprinting, epitope mapping, protein surface mapping, radicals, proteomics, interactomics, trifluoromethylation

Petr Novak
contact affiliation	Institute of Microbiology, The Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic,
contact email	pnovak@biomed.cas.cz
lab head
Zdenek Kukacka
contact affiliation	Institute of Microbiology, The Czech Academy of Sciences
contact email	zdenek.kukacka@biomed.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD027310

PRIDE project URI

• PRIDE
  1. PXD027310
     1. Label: PRIDE project
     2. Name: Fast Fluoroalkylation of Proteins Uncovers Structure and Dynamics of Biological Macromolecules