PXD027309

PXD027309 is an original dataset announced via ProteomeXchange.

Title	Neo-peptides identification in Ewing sarcoma cell lines using proteomics approach
Description	Ewing sarcoma (EwS) is an adolescent and young adult sarcoma characterized by chromosome translocations between members of the FET family of RNA binding proteins and members of the ETS family of transcription factors, the most frequent fusion being EWS-FLI1. EWS-FLI1 acts as a pioneer factor, creating de novo enhancers and activating genes located in the vicinity of EWS-FLI1-bound microsatellite sequences. recent results from our lab indicate that EWS-FLI1, which activates transcription through binding to the DNA at specific sites, can generate fully novel, unconventional transcription units in regions of the genome that are fully quiescent in normal cells (manuscript in preparation). The hypothesis of the project is that the open reading frames (ORFs) of these transcripts may encode peptides presented at the cell surface by HLA class I molecules and hence be recognized as non-self by the immune system. The aim of this study is to detect Ewing-specific neo-peptides/proteins using proteomics approach.
HostingRepository	PRIDE
AnnounceDate	2022-05-12
AnnouncementXML	Submission_2022-05-12_02:48:44.601.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victor Laigle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-14 09:26:39	ID requested
⏵ 1	2022-05-12 02:48:44	announced

Dataset with its publication pending

submitter keyword: Ewing sarcoma, neo-peptides, cell lines, proteomics

Damarys Loew
contact affiliation	Head of the Curie Institute Mass Spectrometry Platform
contact email	damarys.loew@curie.fr
lab head
Victor Laigle
contact affiliation	Mass Spectrometry Proteomics Facility (LSMP) - Institut Curie
contact email	victor.laigle@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD027309

PRIDE project URI

• PRIDE
  1. PXD027309
     1. Label: PRIDE project
     2. Name: Neo-peptides identification in Ewing sarcoma cell lines using proteomics approach