PXD027308

PXD027308 is an original dataset announced via ProteomeXchange.

Title	GSK3-Mediated Phosphorylation of DEK3 Regulates Chromatin Accessibility and Stress Tolerance in Arabidopsis
Description	Chromatin dynamics enables the precise control of transcriptional programs. The balance between restricting and opening of regulatory sequences on the DNA needs to be adjusted to prevailing conditions and is fine-tuned by chromatin remodelling proteins. DEK is an evolutionarily conserved chromatin architectural protein regulating important chromatin-related processes. However, the molecular link between DEK-induced chromatin reconfigurations and upstream signalling events remains unknown. Here, we show that ASKβ/AtSK31 is a salt stress-activated Glycogen Synthase Kinase 3 (GSK3) from Arabidopsis thaliana that phosphorylates DEK3. This specific phosphorylation alters nuclear DEK3 protein complex composition, affects nucleosome occupancy and chromatin accessibility that is translated into changes in gene expression, contributing to salt stress tolerance. These findings reveal that DEK3 phosphorylation is critical for chromatin function and cellular stress response and provide a mechanistic example of how GSK3-based signalling is directly linked to chromatin, facilitating a transcriptional response.
HostingRepository	PRIDE
AnnounceDate	2021-09-14
AnnouncementXML	Submission_2021-09-14_01:02:01.857.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-14 09:26:39	ID requested
⏵ 1	2021-09-14 01:02:02	announced

Waidmann S, Petutschnig E, Rozhon W, Moln, á, r G, Popova O, Mechtler K, Jonak C, GSK3-mediated phosphorylation of DEK3 regulates chromatin accessibility and stress tolerance in Arabidopsis. FEBS J, 289(2):473-493(2022) [pubmed]

submitter keyword: Chromatin / DEK / protein kinase / transcription / salt stress signalling

Claudia Jonak
contact affiliation	Gregor Mendel Institute, Austrian Academy of Sciences, Vienna BioCenter, Dr. Bohr-Gasse 3, Vienna, Austria Current address: AIT Austrian Institute of Technology, Center for Health & Bioresources, Bioresources, Konrad Lorenz Strasse 24, 3430 Tulln, Austria
contact email	claudia.jonak@ait.ac.at
lab head
Richard Imre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027308
     1. Label: PRIDE project
     2. Name: GSK3-Mediated Phosphorylation of DEK3 Regulates Chromatin Accessibility and Stress Tolerance in Arabidopsis