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PXD027302

PXD027302 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of GBP2- and NAB2-interacting proteins in malaria parasites
DescriptionThe quality control and export of mRNA by RNA-binding proteins are necessary for the survival of malaria parasites, which have complex life cycles. Malarial nuclear poly(A) binding protein 2 (NAB2), ALWAYS EARLY (ALY) and serine/arginine-rich (SR) proteins, such as nucleolar protein 3 (NPL3), G-strand binding protein 2 (GBP2) and SR1, are involved in nuclear mRNA export in malaria parasites. However, their functions and cellular localization are not fully understood. In this study, we found that NAB2 and SR1, but not ALY, NPL3 or GBP2, played essential roles in the asexual development of malaria parasites, while GBP2 was involved in gametocyte production. Moreover, GBP2 localized to both the nucleus and cytoplasm of malaria parasites and interacted with the proteins ALBA4, DOZI and CITH, which play roles in translational repression. Our findings suggest that malarial GBP2 may be involved in the recruitment of ALBA4, DOZI and CITH. Immunoprecipitation coupled to mass spectrometry (IP-MS) revealed that PHAX domain-containing protein, an adaptor protein for exportin-1, also interacted with GBP2, suggesting that mRNA export occurs via the PHAX domain-containing protein pathway in malaria parasites. Fluorescence live cell imaging revealed that malarial NAB2 localized at the nuclear periphery and co-localized with NUP205. Moreover, using IP-MS, we found that malarial NAB2 interacted with transportin. RNA immunoprecipitation coupled to RNA sequencing revealed that malarial NAB2 bound directly to 143 mRNAs, including those encoding 40S and 60S ribosomal proteins. This indicates that malarial NAB2 is involved in general mRNA assembly and is shuttled between the nucleus and cytoplasm. Our findings suggest that unique mRNA export and post-transcriptional gene regulation mediated by RNA-binding proteins occur in malaria parasites.
HostingRepositoryPRIDE
AnnounceDate2021-11-03
AnnouncementXMLSubmission_2021-11-03_02:41:59.005.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMamoru Niikura
SpeciesList scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-14 07:34:00ID requested
12021-11-03 02:41:59announced
Publication List
Niikura M, Fukutomi T, Mitobe J, Kobayashi F, Roles and Cellular Localization of GBP2 and NAB2 During the Blood Stage of Malaria Parasites. Front Cell Infect Microbiol, 11():737457(2021) [pubmed]
Keyword List
submitter keyword: LC-MS/MS
Contact List
Mamoru Niikura
contact affiliationKyorin University School of Medicine
contact emailmniikura@ks.kyorin-u.ac.jp
lab head
Mamoru Niikura
contact affiliationDepartment of Infectious Diseases, Kyorin University School of Medicine
contact emailmniikura@ks.kyorin-u.ac.jp
dataset submitter
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Dataset FTP location
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