PXD027284

PXD027284 is an original dataset announced via ProteomeXchange.

Title	NK-cell derived extracellular vesicles LC-MS/MS
Description	In this study we have compared the proteomic profile of extracellular vesicles (EVs) prepared from primary, human NK cells or the human NK cell lines NK-92 and KHYG-1 cultured for 48hrs in serum-free conditions. EVs were harvested from cells either under resting conditions (culture in IL-15) or upon activation (combination of IL-12, IL-15, and IL-18). In addition, primary NK cells were activated in the presence of anti-CD16-coated beads, and EVs harvested after 48hrs. The aim was to compare their ability to target and kill a variety of tumor cell line-derived spheroids
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:36:36.349.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sachin Singh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-13 22:23:48	ID requested
1	2022-10-14 03:50:13	announced
⏵ 2	2023-11-14 07:36:37	announced	2023-11-14: Updated project metadata.

Aarsund M, Segers FM, Wu Y, Inngjerdingen M, Comparison of characteristics and tumor targeting properties of extracellular vesicles derived from primary NK cells or NK-cell lines stimulated with IL-15 or IL-12/15/18. Cancer Immunol Immunother, 71(9):2227-2238(2022) [pubmed]

submitter keyword: NK-92,NK cells, IL-18, CD16, IL-12, KHYG-1, IL-15, LC-MS/MS

Marit Inngjerdingen
contact affiliation	Dept of pharmacology Oslo University Hospital Oslo, Norway
contact email	mariti@medisin.uio.no
lab head
Sachin Singh
contact affiliation	Proteomics core facility, Olso University hospital, Norway
contact email	sachin.kumar.singh@rr-research.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD027284

PRIDE project URI

• PRIDE
  1. PXD027284
     1. Label: PRIDE project
     2. Name: NK-cell derived extracellular vesicles LC-MS/MS