PXD027283

PXD027283 is an original dataset announced via ProteomeXchange.

Title	Identification of molecular machinery mediating ER to lipid droplet protein targeting
Description	Lipid droplets (LDs) are organelles that store lipids and contain important metabolic proteins at their surfaces. The pathway for membrane-embedded hydrophobic proteins to reach the LD surface from the ER is largely unknown. Here, we find that many proteins, including key lipid synthesis and degradation enzymes, are excluded from forming LDs in the ER by the seipin oligomeric complex and target LDs via ER-LD membrane continuities that are established well after LD formation. We utilized systematic, unbiased approaches to identify key components of this ER-to-LD (ERTOLD) pathway. We find that specific membrane-fusion machinery, including membrane fusion regulators (Trs20 and Rab1), a tether (Rint1), and effectors (Syx5, membrin, Bet1, Ykt6), are required for late ERTOLD targeting of GPAT4 and other proteins that utilize this pathway. The fusion machinery components localize to the interface of LDs and the ER, and within the ER, appear to be organized at ER exit sites. Our data therefore uncover a novel mechanism for membrane protein trafficking for ERTOLD targeting via heterotypic organelle fusion between the ER and LDs.
HostingRepository	PRIDE
AnnounceDate	2022-06-28
AnnouncementXML	Submission_2022-06-27_16:21:44.353.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zon Weng Lai
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-13 22:15:24	ID requested
⏵ 1	2022-06-27 16:21:44	announced

Dataset with its publication pending

submitter keyword: protein targeting, protein trafficking, membrane fusion, lipid droplet, endoplasmic reticulum

Tobias Walther
contact affiliation	Harvard T.H. Chan School of Public Health
contact email	twalther@hsph.harvard.edu
lab head
Zon Weng Lai
contact affiliation	Harvard T. H. Chan School of Public Health
contact email	zlai@hsph.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027283
     1. Label: PRIDE project
     2. Name: Identification of molecular machinery mediating ER to lipid droplet protein targeting