PXD027282

PXD027282 is an original dataset announced via ProteomeXchange.

Title	Proteomics informed deconvolution of 3 cell lines
Description	The project aimed to investigate the possibility to use proteomics data to deconvolute cell line proportions in mixed samples. Samples containing either HEK 293, Caco-2, or A549 cells and mixtures of the three cell lines was analysed using the total protein approach. This was then used for proteomics informed deconvolution. The results show that proteome deconvolution provides an effective tool for investigating cellular composition in mixed samples. This was later applied also to in silico mixtures of primary human liver cells and liver tissue. However, those data are presented elsewhere.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:09:33.941.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Niklas Handin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-13 22:14:29	ID requested
1	2023-10-24 12:27:39	announced
⏵ 2	2023-11-14 09:09:40	announced	2023-11-14: Updated project metadata.

Handin N, Yuan D, Ö, lander M, Wegler C, Karlsson C, Jansson-L, ö, fmark R, Hjelmes, æ, th J, Å, sberg A, Lauschke VM, Artursson P, Proteome deconvolution of liver biopsies reveals hepatic cell composition as an important marker of fibrosis. Comput Struct Biotechnol J, 21():4361-4369(2023) [pubmed]

submitter keyword: HEK 293, A549, Caco-2

Per Artursson
contact affiliation	Department of Pharmacy, Uppsala University, SE-75123 Uppsala, Sweden.
contact email	Per.Artursson@farmaci.uu.se
lab head
Niklas Handin
contact affiliation	Uppsala University, Department of Pharmacy, Drug Delivery
contact email	Niklas.Handin@farmaci.uu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD027282

PRIDE project URI

• PRIDE
  1. PXD027282
     1. Label: PRIDE project
     2. Name: Proteomics informed deconvolution of 3 cell lines