PXD027280

PXD027280 is an original dataset announced via ProteomeXchange.

Title	Notch Signaling Enhances Bone Regeneration in the Zebrafish Mandible
Description	Loss or damage to the mandible due to trauma, treatment of oral malignancies, and other diseases is currently treated using bone grafting techniques that suffer from numerous shortcomings and contraindications. Zebrafish naturally heal large injuries to their mandibular bone, and thus offer an opportunity to understand how to boost intrinsic healing ability. Using a novel her6:mCherry Notch reporter, we show that canonical Notch signaling is induced during the initial stages of cartilage callus formation in both mesenchymal cells and chondrocytes. We also show that modulation of Notch signaling during the initial postoperative period results in lasting changes to regenerate bone quantity one month later. Notch signaling is required for mandibular bone healing, as pharmacological inhibition of Notch signaling blocks cartilage callus formation and results in non-union. Conversely, conditional transgenic activation of Notch signaling accelerates regenerative ossification. Mechanistically, we report that postoperative Notch signaling regulates multiple phases of chondroid regeneration and patterns callus metabolic landscape. Given conserved functions of Notch signaling in bone repair across vertebrates, we propose that targeted activation of Notch signaling during the early phases of bone healing may have therapeutic value.
HostingRepository	PRIDE
AnnounceDate	2022-03-07
AnnouncementXML	Submission_2022-03-07_13:57:15.277.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027280
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jeremy Balsbaugh
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-13 22:12:51	ID requested
⏵ 1	2022-03-07 13:57:16	announced

Kraus JM, Giovannone D, Rydzik R, Balsbaugh JL, Moss IL, Schwedler JL, Bertrand JY, Traver D, Hankenson KD, Crump JG, Youngstrom DW, Notch signaling enhances bone regeneration in the zebrafish mandible. Development, 149(5):(2022) [pubmed]

submitter keyword: Notch signaling, proteomics, mass spectrometry, Q Exactive HF, FASP, mandible, bone, chondrocytes, zebrafish

Jeremy L. Balsbaugh
contact affiliation	Proteomics & Metabolomics Facility, Center for Open Research Resources & Equipment University of Connecticut Storrs, CT 06269
contact email	jeremy.balsbaugh@uconn.edu
lab head
Jeremy Balsbaugh
contact affiliation	University of Connecticut
contact email	jeremy.balsbaugh@uconn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD027280

PRIDE project URI

• PRIDE
  1. PXD027280
     1. Label: PRIDE project
     2. Name: Notch Signaling Enhances Bone Regeneration in the Zebrafish Mandible