PXD027267

PXD027267 is an original dataset announced via ProteomeXchange.

Title	Proxiome at the 40S ribosomal head region according to proximity labeling MS experiments
Description	The in vivo labeling technique Biotin identification (BioID, Roux et al., 2012) was used to capture proteins in the microenvironments of the ribosomal proteins Rps3 (uS3) and Rps20 (us10) in Saccharomyces cerevisiae. Biotinylated proteins were captured from cell lysates using affinity purification and subjected to SDS-PAGE followed by in-gel digestion with trypsin. Liquid chromatography-mass spectrometry (LC-MS) was performed to identify and relatively quantify peptides and thus proteins. Relative quantification of proteins was based on stable isotope labeling with amino acids in cell culture (SILAC). To account for putative differences in the expression levels of the enriched proteins, an input control was analyzed as well. To this end, an aliquot of the cell lysate was taken prior to protein enrichment and analyzed as well. Beyond the known and expected interaction partners of Rps3 and Rps20, we identified proteins not known to co-localize with them. Moreover, we could also observe changes in their environment when the WD40-repeat protein Asc1/RACK1 was absent from the head region.
HostingRepository	PRIDE
AnnounceDate	2021-11-10
AnnouncementXML	Submission_2021-11-10_07:56:42.460.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Valerius
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-13 05:15:53	ID requested
⏵ 1	2021-11-10 07:56:42	announced

Dataset with its publication pending

submitter keyword: Rps3, uS3, Rps20, uS10, BioID, BirA*, SILAC, Saccharomyces cerevisiae, ribosome

Oliver Valerius
contact affiliation	Georg-August-University Institute of Microbiology & Genetics Department of Mol. Microbiol. & Genetics Grisebachstr. 8 Göttingen
contact email	ovaleri@gwdg.de
lab head
Oliver Valerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027267
     1. Label: PRIDE project
     2. Name: Proxiome at the 40S ribosomal head region according to proximity labeling MS experiments