PXD027260

PXD027260 is an original dataset announced via ProteomeXchange.

Title	Screening of host proteins interacting with African swine fever virus MGF360-9L
Description	To identify the potential host protein binding to African swine fever virus MGF360-9L, the 3 × FLAG tag MGF360-9L plasmid and empty FLAG were transfected into PK-15 cells for co-immunoprecipitation and LC-MS analysis. The cell lysates were immunoprecipitated using anti-FLAG antibody. Immunoprecipitation samples of cells transfected with empty FLAG were used as negative controls to eliminate non-specific interactions. LC-MS identified the interaction between MGF360-9L and PK-15 cell proteome. The final MGF360-9L binding protein data eliminated the nonspecific interaction through empty FLAG control. The interactions that remained were analyzed by significance analysis of interactome. Finally, 268 cellular proteins interacting with MGF360-9L were identified.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:16:34.710.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yanzhen Zhao
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-12 22:18:03	ID requested
⏵ 1	2022-02-17 05:16:35	announced

Yang B, Zhang D, Shi X, Shen C, Hao Y, Zhang T, Yang J, Yuan X, Chen X, Zhao D, Cui H, Li D, Zhu Z, Tian H, Yang F, Zheng H, Zhang K, Liu X, Construction, Identification and Analysis of the Interaction Network of African Swine Fever Virus MGF360-9L with Host Proteins. Viruses, 13(9):(2021) [pubmed]

submitter keyword: African swine fever virus

MGF360-9L

protein interaction

liquid chromatography-mass spectrometry

Bo Yang
contact affiliation	Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping No.1, Lanzhou, Gansu, 730046
contact email	2019107002@njau.edu.cn
lab head
yanzhen Zhao
contact affiliation	shanghai
contact email	449096780@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027260

PRIDE project URI

• PRIDE
  1. PXD027260
     1. Label: PRIDE project
     2. Name: Screening of host proteins interacting with African swine fever virus MGF360-9L