PXD027260 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Screening of host proteins interacting with African swine fever virus MGF360-9L |
Description | To identify the potential host protein binding to African swine fever virus MGF360-9L, the 3 × FLAG tag MGF360-9L plasmid and empty FLAG were transfected into PK-15 cells for co-immunoprecipitation and LC-MS analysis. The cell lysates were immunoprecipitated using anti-FLAG antibody. Immunoprecipitation samples of cells transfected with empty FLAG were used as negative controls to eliminate non-specific interactions. LC-MS identified the interaction between MGF360-9L and PK-15 cell proteome. The final MGF360-9L binding protein data eliminated the nonspecific interaction through empty FLAG control. The interactions that remained were analyzed by significance analysis of interactome. Finally, 268 cellular proteins interacting with MGF360-9L were identified. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_05:16:34.710.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | yanzhen Zhao |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; |
ModificationList | acetylated residue; monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-12 22:18:03 | ID requested | |
⏵ 1 | 2022-02-17 05:16:35 | announced | |
Publication List
Yang B, Zhang D, Shi X, Shen C, Hao Y, Zhang T, Yang J, Yuan X, Chen X, Zhao D, Cui H, Li D, Zhu Z, Tian H, Yang F, Zheng H, Zhang K, Liu X, Construction, Identification and Analysis of the Interaction Network of African Swine Fever Virus MGF360-9L with Host Proteins. Viruses, 13(9):(2021) [pubmed] |
Keyword List
submitter keyword: African swine fever virus |
MGF360-9L |
protein interaction |
liquid chromatography-mass spectrometry |
Contact List
Bo Yang |
contact affiliation | Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping No.1, Lanzhou, Gansu, 730046 |
contact email | 2019107002@njau.edu.cn |
lab head | |
yanzhen Zhao |
contact affiliation | shanghai |
contact email | 449096780@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027260
- Label: PRIDE project
- Name: Screening of host proteins interacting with African swine fever virus MGF360-9L