PXD027257

PXD027257 is an original dataset announced via ProteomeXchange.

Title	Cellular interactome of HSV-1 pUL21
Description	The herpes simplex virus (HSV)-1 protein pUL21 is essential for efficient virus replication and dissemination. While pUL21 has been shown to promote multiple steps of virus assembly and spread, the molecular basis of its function remained unclear. Here we identify that pUL21 is a virus-encoded adaptor of protein phosphatase 1 (PP1). pUL21 directs the dephosphorylation of cellular and virus proteins, including components of the viral nuclear egress complex, and we define a conserved non-canonical linear motif in pUL21 that is essential for PP1 recruitment. In vitro evolution experiments reveal that pUL21 directly antagonises the activity of the virus-encoded kinase pUS3, with growth and spread of pUL21 PP1-binding mutant viruses being restored when pUS3 activity is disrupted. This study shows that virus-directed phosphatase activity is essential for efficient herpesvirus assembly and spread, highlighting the fine balance between kinase and phosphatase activity required for optimal virus replication.
HostingRepository	PRIDE
AnnounceDate	2021-08-05
AnnouncementXML	Submission_2021-08-05_05:09:47.976.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephen Graham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	isotope labeled residue; monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-12 22:08:53	ID requested
⏵ 1	2021-08-05 05:09:48	announced

Dataset with its publication pending

submitter keyword: Herpes simplex virus 1, herpesvirus, protein phosphatase 1, PP1, COL4A3BP, CERT, SILAC

Stephen Charles Graham
contact affiliation	Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP
contact email	scg34@cam.ac.uk
lab head
Stephen Graham
contact affiliation	Department of Pathology University of Cambridge
contact email	scg34@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027257
     1. Label: PRIDE project
     2. Name: Cellular interactome of HSV-1 pUL21