PXD027254

PXD027254 is an original dataset announced via ProteomeXchange.

Title	Data for: Short-chain fatty acids activate acetyltransferase p300/CBP
Description	The short-chain fatty acids (SCFAs) acetate, propionate, and butyrate are produced in large quantities by the gut microbiome and contribute to a wide array of physiological processes. While the underlying mechanisms are largely unknown, many effects of SCFAs have been traced to changes in epigenetic state. Here, we systematically investigate how SCFAs alter the epigenome. Using quantitative proteomics of histone modification states, we identified rapid and sustained increases in histone acetylation after addition of butyrate or propionate, but not acetate. While decades of prior observations would have suggested that hyperacetylation induced by SCFAs are attributed to inhibition of histone deacetylases (HDACs), we found that propionate and butyrate instead activate the acetyltransferase p300/CBP. Propionate and butyrate are rapidly converted to the corresponding acyl-CoAs which are then used by p300/CBP to catalyze auto-acylation of the autoinhibitory loop, activating the enzyme for histone/protein acetylation. This data challenges the long-held belief that SCFAs mainly regulate chromatin by inhibiting HDACs, and instead reveals a previously unappreciated mechanism of HAT activation that can explain how even low levels of SCFAs can alter epigenomes.
HostingRepository	MassIVE
AnnounceDate	2021-10-26
AnnouncementXML	Submission_2021-10-26_12:49:39.276.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sydney Thomas
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Acetyl; Propionyl; Butyryl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-12 10:35:49	ID requested
⏵ 1	2021-10-26 12:49:40	announced

Thomas SP, Denu JM, Short-chain fatty acids activate acetyltransferase p300. Elife, 10():(2021) [pubmed]

submitter keyword: short-chain fatty acids, metabolomics, proteomics, p300/CBP

John Denu
contact affiliation	University of Wisconsin - Madison
contact email	john.denu@wisc.edu
lab head
Sydney Thomas
contact affiliation	University of Wisconsin - Madison
contact email	sthomas28@wisc.edu
dataset submitter

MassIVE dataset URI

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