PXD027251 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Insights into transcriptome surveillance and alternative splicing regulation by the EJC-associated splicing factor RNPS1 |
Description | Alternative splicing of pre-mRNAs increases the potential for regulation and complexity of gene expression. The exon junction complex (EJC) and its associated splicing factor RNPS1 were recently shown to suppress mis-splicing resulting from the usage of cryptic and reconstituted 5’ and 3’ splice sites in the vicinity of the EJC. Here, we aimed to further investigate the mechanisms underlying splicing regulation by RNPS1. A transcriptome-wide analysis identified hundreds of splice events affected by the knockdown (KD) of RNPS1 in HeLa cells. These included alternative splice site usage as well as intron retention, exon skipping and inclusion. However, only a fraction of these RNPS1-dependent splice events was fully or partially rescued by the expression of the RNPS1 RRM. These results indicated that another domain of RNPS1 is involved in the regulation of the majority of splicing events. Deletion experiments revealed that the N-terminus and S-domain, and in particular the C-terminus of RNPS1 strongly regulate these events. Several splicing factors, including SR proteins and U1 snRNP components, were strongly reduced in the interactome of RNPS1 lacking the C terminus. We conclude that RNPS1 interacts with many splicing factors to direct the assembly of EJC-dependent and-independent splicing complexes. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-31 |
AnnouncementXML | Submission_2022-05-31_02:36:47.781.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Prerana Wagle |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-12 08:17:58 | ID requested | |
⏵ 1 | 2022-05-31 02:36:48 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: RNPS1, mRNA splicing, exon junction complex |
Contact List
Niels H. Gehring |
contact affiliation | Institute for Genetics, UzK, Cologne, Germany |
contact email | ngehring@uni-koeln.de |
lab head | |
Prerana Wagle |
contact affiliation | CECAD Research Center |
contact email | proteomics-facility@uni-koeln.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD027251 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027251
- Label: PRIDE project
- Name: Insights into transcriptome surveillance and alternative splicing regulation by the EJC-associated splicing factor RNPS1