PXD027246

PXD027246 is an original dataset announced via ProteomeXchange.

Title	Increased cellular detoxification, cytoskeletal activities and protein transport explain physiological stress in a lagoon sponge
Description	Tropical lagoon-inhabiting organisms live in highly irradiated ecosystems and are particularly susceptible to thermal stress resulting from climate change. However, despite living close to their thermal maxima, stress response mechanisms found in these organisms are poorly understood. We used a novel physiological-proteomic approach for sponges to describe the stress response mechanisms of the lagoon-inhabiting sponge Amphimedon navalis, when exposed to elevated seawater temperatures of +2 oC and +4 oC relative to a 26 oC ambient temperature for four weeks. After four weeks of thermal exposure, the buoyant weight of the sponge experienced a significant decline, while its pumping rates and oxygen consumption rates significantly increased. Proteome dynamics revealed 50 differentially abundant proteins in sponges exposed to elevated temperature, suggesting that shifts in the sponge proteome were potential drivers of physiological dysfunction. Thermal stress promoted an increase in detoxification proteins, such as catalase and glutathione-S-transferase, suggesting that an excess of reactive oxygen species in sponge cells were likely responsible for the significant increase in oxygen consumption. Elevated temperature also disrupted cellular growth and cell proliferation, promoting the loss of sponge biomass, and the high abundance of multiple alpha-tubulin chain proteins also indicated an increase in cytoskeletal activities within sponge cells, which may have induced the increase in sponge pumping rate. Our results show that sustained thermal exposure in susceptible lagoonal sponges may induce significant disruption of cellular homeostasis leading to physiological dysfunction, and that a combined physiological-proteomic approach may provide new insights into physiological functions and cellular processes occurring in sponges.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:15.201.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clinton Oakley
SpeciesList	scientific name: Amphimedon; NCBI TaxID: 178513;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-12 08:17:29	ID requested
1	2021-11-15 09:26:39	announced
⏵ 2	2023-11-14 08:53:15	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics, physiology, lagoon, thermal stress,Sponge, climate change

Simon K Davy
contact affiliation	School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand
contact email	simon.davy@vuw.ac.nz
lab head
Clinton Oakley
contact affiliation	Victoria University of Wellington
contact email	clint.oakley@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD027246

PRIDE project URI

• PRIDE
  1. PXD027246
     1. Label: PRIDE project
     2. Name: Increased cellular detoxification, cytoskeletal activities and protein transport explain physiological stress in a lagoon sponge