PXD027239

PXD027239 is an original dataset announced via ProteomeXchange.

Title	Metabolic labeling of NMT substrates by photoactivatable/clickable myristate probes X3/X8/X10
Description	Efficiency of labelling by photoactivatable/clickable myristate probes X3/X8/X10 is compared to the positive control probe YnMyr. Additionally, negative controls for each probe were obtained by treatment with NMT ihibitor IMP-366 (DDD85464). The resulting data indicates efficient labeling of NMT substrates by photoactivatable probes that is NMT-dependent.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:38:44.403.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roman Fedoryshchak
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	myristoylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-12 01:43:20	ID requested
1	2023-03-10 23:47:52	announced
⏵ 2	2023-11-14 08:38:45	announced	2023-11-14: Updated project metadata.

Fedoryshchak RO, Gorelik A, Shen M, Shchepinova MM, P, é, rez-Dorado I, Tate EW, Discovery of lipid-mediated protein-protein interactions in living cells using metabolic labeling with photoactivatable clickable probes. Chem Sci, 14(9):2419-2430(2023) [pubmed]

submitter keyword: NMT, diazirine, metabolic labeling, myristoylation, photocrosslinking

Edward Tate
contact affiliation	Professor of Chemical Biology, Imperial College London Satellite Group Leader, Francis Crick Institute Founder & SAB, Myricx Pharma Ltd Room 301B, Molecular Sciences Research Hub, White City Campus 82 Wood Lane, London W12 0BZ, UK
contact email	e.tate@imperial.ac.uk
lab head
Roman Fedoryshchak
contact affiliation	The Francis Crick Institute
contact email	roman.fedoryshchak@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD027239

PRIDE project URI

• PRIDE
  1. PXD027239
     1. Label: PRIDE project
     2. Name: Metabolic labeling of NMT substrates by photoactivatable/clickable myristate probes X3/X8/X10