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PXD027237

PXD027237 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of acetylation-regulated interactors of the human CCR4-NOT complex
DescriptionThe evolutionarily conserved CCR4-NOT complex acts as a central player in the control of mRNA turnover and mediates accelerated mRNA degradation upon histone deacetylase (HDAC) inhibition. Here, we explored acetylation-induced changes in the composition of the CCR4-NOT complex by purification of the endogenously tagged scaffold subunit NOT1 and identified ring finger protein 219 (RNF219) as an acetylation-regulated cofactor. We provide evidence that RNF219 is an active RING-type E3 ubiquitin ligase which stably associates with the CCR4-NOT complex via the NOT9 module through a short linear motif located in the C-terminal low-complexity region of RNF219. Using a reconstituted six-subunit human CCR4-NOT complex, we demonstrate that RNF219 acts as an inhibitor of deadenylation. Accordingly, our transcriptome-wide mRNA half-life measurements reveal that RNF219 attenuates global mRNA turnover in cells, independently of its E3 ligase activity. Our results establish RNF219 as a negative regulator of CCR4-NOT-mediated mRNA deadenylation, whose loss upon HDAC inhibition contributes to accelerated mRNA turnover.
HostingRepositoryPRIDE
AnnounceDate2021-12-20
AnnouncementXMLSubmission_2021-12-21_03:36:59.034.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Ruppert
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-12 01:43:08ID requested
12021-12-20 11:44:03announced
22021-12-21 03:37:00announced2021-12-21: Updated publication reference for PubMed record(s): 34887419.
Publication List
Poetz F, Corbo J, Levdansky Y, Spiegelhalter A, Lindner D, Magg V, Lebedeva S, Schweiggert J, Schott J, Valkov E, Stoecklin G, RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation. Nat Commun, 12(1):7175(2021) [pubmed]
Keyword List
submitter keyword: CCR4-NOT complex, protein acetylation, mRNA turnover, deadenylation, E3 ubiquitin ligase, RNF219
Contact List
Thomas Ruppert
contact affiliationUniversity Heidelberg ZMBHCore Facility for Mass Spectrometry and Proteomics Im Neuenheimer Feld 282 69120 Heidelberg Germany
contact emailt.ruppert@zmbh.unni-heidelberg.de
lab head
Thomas Ruppert
contact affiliationZMBH, Im Neuenheimer Feld 282, 69122 Heidelberg
contact emailt.ruppert@zmbh.uni-heidelberg.de
dataset submitter
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