PXD027236

PXD027236 is an original dataset announced via ProteomeXchange.

Title	Proteomic response of Deinococcus radiodurans to short-term real microgravity during parabolic flight
Description	As plans for future space exploration are becoming more ambitious, a better understanding of all factors affecting humans, plants, and microorganisms in space is necessary. Microgravity is an important variable in outer space and understanding the short- and long- term effects of microgravity on cellular processes will be important to minimize its negative effects on the physiology of any organism. Gravitational force has had an important role in the development of life on Earth, and short- and long-term changes in perceived gravitational force can induce notable changes within cells. Deinococcus radiodurans is the gram-positive bacterium that is best known for its extreme resistance to UV-C and gamma radiation, oxidation stress and desiccation, which has led to increased interest in this species in the context of astrobiology. The present study aimed to elucidate the short-term proteomic response of this species to real microgravity during parabolic flight. Overnight cultures were subjected to microgravity during a single parabola, and metabolic activity quenched using methanol. Proteins were extracted and subsequently measured using HPLC ESI MS/MS. Results indicated multiple affected processes in the cell envelope of D. radiodurans, such as increased peptidoglycan synthesis and altered S-layer activities. Energy metabolism upregulation and increased activity of DNA repair pathways could indicate increased endogenous ROS production that contributes to the stress response. The present study shows that the D. radiodurans proteome reacts to real microgravity within seconds. Differential expression patterns in response to microgravity show similarities to previously reported stress responses, thus the present results could be used as basis for future research aiming to better understand the complex regulatory processes underpinning stress management in D. radiodurans.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:14:29.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karlis Moors
SpeciesList	scientific name: Deinococcus radiodurans R1; NCBI TaxID: 243230;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-12 01:42:54	ID requested
⏵ 1	2022-02-17 05:14:30	announced

Moors KA, Ott E, Weckwerth W, Milojevic T, to Short-Term Real Microgravity during Parabolic Flight Reveals Altered Abundance of Proteins Involved in Stress Response and Cell Envelope Functions. Life (Basel), 12(1):(2021) [pubmed]

submitter keyword: parabolic flight, bacteria, microgravity, proteomics, outer space

Dr Tetyana Milojevic
contact affiliation	Space Biochemistry Group, Department of Biophysical Chemistry, University of Vienna, Vienna, Austria
contact email	tetyana.milojevic@univie.ac.at
lab head
Karlis Moors
contact affiliation	Vienna University
contact email	kamoors@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027236

PRIDE project URI

• PRIDE
  1. PXD027236
     1. Label: PRIDE project
     2. Name: Proteomic response of Deinococcus radiodurans to short-term real microgravity during parabolic flight