PXD027230

PXD027230 is an original dataset announced via ProteomeXchange.

Title	Exportin 1 modulates lifespan by regulating nucleolar dynamics via the autophagy protein LGG-1/GABARAP
Description	Altered nucleolar and ribosomal dynamics are key hallmarks of aging, but their regulation remains unclear. Building on the knowledge that the conserved nuclear export receptor Exportin 1 (XPO-1/XPO1) modulates proteostasis and lifespan, we systematically analyzed the impact of nuclear export on protein metabolism. Using transcriptomic and subcellular proteomic analyses in nematodes, we demonstrate that XPO-1 modulates the nucleo-cytoplasmic distribution of key proteins involved in nucleolar dynamics and ribosome function, including fibrillarin (FIB-1/FBL) and RPL-11 (RPL11). Silencing XPO-1 led to marked reduction in global translation, which was accompanied by decreased nucleolar size and lower fibrillarin levels. A targeted screen of known proteostatic mediators revealed that the autophagy protein LGG-1/GABARAP modulates nucleolar size by regulating RPL-11 levels, linking specific protein degradation to ribosome metabolism. Altogether, our study reveals that nucleolar size, and consequently lifespan, are regulated by LGG-1/GABARAP via ribosome protein surveillance.
HostingRepository	PRIDE
AnnounceDate	2026-02-23
AnnouncementXML	Submission_2026-02-22_18:38:26.862.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027230
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Taewook Kang
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: NEWT:6239;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-12 01:42:02	ID requested
⏵ 1	2026-02-22 18:38:27	announced

10.1126/sciadv.abj1604;

10.6019/PXD027230;

Kumar AV, Kang T, Thakurta TG, Ng C, Rogers AN, Larsen MR, Lapierre LR, Exportin 1 modulates life span by regulating nucleolar dynamics via the autophagy protein LGG-1/GABARAP. Sci Adv, 8(13):eabj1604(2022) [pubmed]

submitter keyword: autophagy, proteomics,aging, proteostasis

Louis R Lapierre
contact affiliation	Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 185 Meeting St., Providence, Rhode Island 02912, USA
contact email	louis_lapierre@brown.edu
lab head
Taewook Kang
contact affiliation	University of Southern Denmark
contact email	taewookk@bmb.sdu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027230
     1. Label: PRIDE project
     2. Name: Exportin 1 modulates lifespan by regulating nucleolar dynamics via the autophagy protein LGG-1/GABARAP