PXD027221

PXD027221 is an original dataset announced via ProteomeXchange.

Title	Treatment of human K562 chronic myelogenous leukemia cells with NK3.3-derived extracellular vesicles
Description	Our study investigates the features and anti-tumor function of extracellular vesicles (EVs) isolated from our well described NK cell line - NK3.3 - the only published clonal, normal human cell line to date. Natural killer (NK) cells, critical for proper immune function, destroy tumor cells primarily through the release of granules containing potent cytolytic molecules. NK cells also release these molecules within membrane-bound exosomes and microvesicles collectively known as EVs. We show that NK3.3 EVs contain the cytolytic molecules perforin, granzymes A and B, and granulysin, and an array of common EV proteins. We previously showed that the E3 ubiquitin ligase identified by our laboratory, natural killer lytic-associated molecule (NKLAM/RNF19b), is localized to NK granules and is essential for maximal NK killing; our current study shows NKLAM situated within the NK3.3 EV membrane. We demonstrate that treatment of an array of hematopoietic and non-hematopoietic tumor cell lines with NK3.3 EVs inhibits proliferation and induces apoptosis and cell death while leaving normal cells unaffected. The anti-tumor effects of NK3.3EVs were evaluated via proteomic analysis of K562 leukemia cells treated with NK3.3-derived EVs compared to treatment with non-cytotoxic HEK293 cell-derived EVs or PBS. The results of this analysis suggest strong inhibitory regulation of cell cycle- and cell survival-associated proteins. Taken together, our study suggests that NK3.3 EVs have the potential to be effective immunotherapeutic agents.
HostingRepository	PRIDE
AnnounceDate	2021-09-07
AnnouncementXML	Submission_2021-09-07_04:16:16.541.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027221
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Allyson Cochran
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-11 22:35:20	ID requested
⏵ 1	2021-09-07 04:16:17	announced

Cochran AM, Kornbluth J, Extracellular Vesicles From the Human Natural Killer Cell Line NK3.3 Have Broad and Potent Anti-Tumor Activity. Front Cell Dev Biol, 9():698639(2021) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: natural killer cell, extracellular vesicle, NKLAM, RNF19b, NK3.3, ubiquitin ligase, leukemia, exosome, microvesicle, extracellular vesicles

Jacki Kornbluth
contact affiliation	Department of Pathology, School of Medicine, Saint Louis University, United States, St. Louis VA Medical Center(VHA), United States
contact email	jacki.kornbluth@health.slu.edu
lab head
Allyson Cochran
contact affiliation	Saint Louis University - Department of Pathology
contact email	allyson.cochran@slu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD027221

PRIDE project URI

• PRIDE
  1. PXD027221
     1. Label: PRIDE project
     2. Name: Treatment of human K562 chronic myelogenous leukemia cells with NK3.3-derived extracellular vesicles