PXD027216

PXD027216 is an original dataset announced via ProteomeXchange.

Title	Distinct Stress Response to Mitochondrial Inter-membrane Space and Matrix Proteotoxic Stress are Preferentially Modulated by TOM complex and Vms1, respectively
Description	Double-membrane-bound architecture of mitochondria is essential for its ATP synthesis function; simultaneously such structure sub-divides the organelle into inter-membrane space (IMS) and matrix. IMS and matrix are inherently different in protein folding milieu due to their contrasting oxido-reductive environments and distinctly different protein quality control (PQC) machineries. By inducing proteotoxic stress limited to IMS or matrix using varied stressor proteins, we decipher distinct cellular response to IMS and matrix stress. IMS stress leads to specific upregulation of IMS-resident chaperones and TOM complex components. In contrast, matrix stress leads to specific upregulation of matrix- chaperones and cytosolic PQC components. We report that cells respond to mitochondrial stress by an adaptive mechanism by adjourning mitochondrial respiration while upregulating glycolysis as a compensatory pathway. By systematic genetic interaction, we show that TOM complex components act as specific modulators of IMS-stress response while Vms1 preferentially modulates the matrix stress response
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:49:13.149.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Swasti Raychaudhuri
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-09 08:24:21	ID requested
1	2024-07-08 23:57:58	announced
⏵ 2	2024-10-22 06:49:14	announced	2024-10-22: Updated project metadata.

Narayana Rao KB, Pandey P, Sarkar R, Ghosh A, Mansuri S, Ali M, Majumder P, Ranjith Kumar K, Ray A, Raychaudhuri S, Mapa K, Stress Responses Elicited by Misfolded Proteins Targeted to Mitochondria. J Mol Biol, 434(12):167618(2022) [pubmed]

10.1016/j.jmb.2022.167618;

submitter keyword: Proteostasis, Chaperones,Mitochondrial Unfolded Protein Response, Proteotoxic stress, Protein misfolding, Stress Response, Ribosome Quality Control, Vms1

Swasti Raychaudhuri
contact affiliation	CSIR-CCMB
contact email	rcswasti@ccmb.res.in
lab head
Swasti Raychaudhuri
contact affiliation	CSIR-Centre for Cellular and Molecular Biology
contact email	rcswasti@ccmb.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD027216

PRIDE project URI

• PRIDE
  1. PXD027216
     1. Label: PRIDE project
     2. Name: Distinct Stress Response to Mitochondrial Inter-membrane Space and Matrix Proteotoxic Stress are Preferentially Modulated by TOM complex and Vms1, respectively