PXD027214

PXD027214 is an original dataset announced via ProteomeXchange.

Title	A Composite DNA Element that Functions as a Maintainer Required for Epigenetic Inheritance of Heterochromatin
Description	Epigenetic inheritance of heterochromatin requires DNA sequence-independent propagation mechanisms, coupling to RNAi, or input from DNA sequence, but how DNA contributes to inheritance is not understood. Here, we identify a DNA element (termed “maintainer”) that is sufficient for epigenetic inheritance of preexisting histone H3 lysine 9 methylation (H3K9me) and heterochromatin in Schizosaccharomyces pombe, but cannot establish de novo gene silencing in wild-type cells. This maintainer is a composite DNA element with binding sites for the Atf1/Pcr1 and Deb1 transcription factors and the Origin Recognition Complex (ORC), located within a 130-base pair region, and can be converted to a silencer in cells with lower rates of H3K9me turnover, suggesting that it participates in recruiting the H3K9 methyltransferase Clr4/Suv39h. These results suggest that, in the absence of RNAi, histone H3K9me is only heritable when it can collaborate with maintainer-associated DNA-binding proteins that help recruit the enzyme responsible for its epigenetic deposition.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:31:35.673.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-09 08:24:20	ID requested
1	2022-10-14 03:23:38	announced
⏵ 2	2023-11-14 07:31:37	announced	2023-11-14: Updated project metadata.

Wang X, Paulo JA, Li X, Zhou H, Yu J, Gygi SP, Moazed D, A composite DNA element that functions as a maintainer required for epigenetic inheritance of heterochromatin. Mol Cell, 81(19):3979-3991.e4(2021) [pubmed]

submitter keyword: Maintainer, H3K9me, methylation pombe, Heterochromatin

Danesh Moazed
contact affiliation	Department of Cell Biology Harvard Medical School Boston, MA USA
contact email	danesh_moazed@hms.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD027214

PRIDE project URI

• PRIDE
  1. PXD027214
     1. Label: PRIDE project
     2. Name: A Composite DNA Element that Functions as a Maintainer Required for Epigenetic Inheritance of Heterochromatin