PXD027214 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Composite DNA Element that Functions as a Maintainer Required for Epigenetic Inheritance of Heterochromatin |
Description | Epigenetic inheritance of heterochromatin requires DNA sequence-independent propagation mechanisms, coupling to RNAi, or input from DNA sequence, but how DNA contributes to inheritance is not understood. Here, we identify a DNA element (termed “maintainer”) that is sufficient for epigenetic inheritance of preexisting histone H3 lysine 9 methylation (H3K9me) and heterochromatin in Schizosaccharomyces pombe, but cannot establish de novo gene silencing in wild-type cells. This maintainer is a composite DNA element with binding sites for the Atf1/Pcr1 and Deb1 transcription factors and the Origin Recognition Complex (ORC), located within a 130-base pair region, and can be converted to a silencer in cells with lower rates of H3K9me turnover, suggesting that it participates in recruiting the H3K9 methyltransferase Clr4/Suv39h. These results suggest that, in the absence of RNAi, histone H3K9me is only heritable when it can collaborate with maintainer-associated DNA-binding proteins that help recruit the enzyme responsible for its epigenetic deposition. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_07:31:35.673.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joao Paulo |
SpeciesList | scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690; |
ModificationList | monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-09 08:24:20 | ID requested | |
1 | 2022-10-14 03:23:38 | announced | |
⏵ 2 | 2023-11-14 07:31:37 | announced | 2023-11-14: Updated project metadata. |
Publication List
Wang X, Paulo JA, Li X, Zhou H, Yu J, Gygi SP, Moazed D, A composite DNA element that functions as a maintainer required for epigenetic inheritance of heterochromatin. Mol Cell, 81(19):3979-3991.e4(2021) [pubmed] |
Keyword List
submitter keyword: Maintainer, H3K9me, methylation pombe, Heterochromatin |
Contact List
Danesh Moazed |
contact affiliation | Department of Cell Biology Harvard Medical School Boston, MA USA |
contact email | danesh_moazed@hms.harvard.edu |
lab head | |
Joao Paulo |
contact affiliation | Harvard Medical School |
contact email | joao_paulo@post.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027214
- Label: PRIDE project
- Name: A Composite DNA Element that Functions as a Maintainer Required for Epigenetic Inheritance of Heterochromatin