PXD027213

PXD027213 is an original dataset announced via ProteomeXchange.

Title	Structural architecture and function of the human NALCN channelosome
Description	Chemical cross-linking coupled to mass spectrometry was used to study the subunit organization of the NALCN channelosome consisting of the proteins NALCN, FAM155A, UNC79 and UNC80. Cross-linking data was generated using the reagents disuccinimidyl suberate (DSS), pimelic dihydrazide (PDH), and 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride, and was analyzed in combination with data from cryo-electron microscopy.
HostingRepository	PRIDE
AnnounceDate	2021-11-23
AnnouncementXML	Submission_2022-03-14_04:33:46.202.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Leitner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-09 06:18:43	ID requested
1	2021-11-23 07:27:35	announced
⏵ 2	2022-03-14 04:33:47	announced	2022-03-14: Updated publication reference for PubMed record(s): 34929720.

Kschonsak M, Chua HC, Weidling C, Chakouri N, Noland CL, Schott K, Chang T, Tam C, Patel N, Arthur CP, Leitner A, Ben-Johny M, Ciferri C, Pless SA, Payandeh J, Structural architecture of the human NALCN channelosome. Nature, 603(7899):180-186(2022) [pubmed]

submitter keyword: Chemical cross-linking

DSS

PDH

DMTMM

ion channel

sodium leak channel

NALCN

Alexander Leitner
contact affiliation	ETH Zurich, Institute of Molecular Systems Biology, Zurich, Switzerland
contact email	leitner@imsb.biol.ethz.ch
lab head
Alexander Leitner
contact affiliation	ETH Zurich, Institute of Molecular Systems Biology
contact email	leitner@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD027213

PRIDE project URI

• PRIDE
  1. PXD027213
     1. Label: PRIDE project
     2. Name: Structural architecture and function of the human NALCN channelosome