PXD027208

PXD027208 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of extracellular proteins in Acinetobacter baumannii in sessile mode of growth
Description	Here, we have investigated, for the first time, the serine, threonine and tyrosine phosphoproteome in the extracellular medium of A. baumannii during biofilm mode of growth for two strains: the reference strain ATCC 17978 and the virulent multi-drug resistant strain AB0057, a clinical isolate from a bloodstream infection. Overall, a total of 109 and 146 phosphorylated proteins were identified for ATCC 17978 and AB0057, respectively. We also showed that one specific phosphorylation site was essential for the activity of the A. baumannii type VI secretion system. This provides the first global phosphosecretome profiling of A. baumannii. This analysis represents a promising starting point for further investigations on the biological role of phosphorylation in A. baumannii.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:20:25.025.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julie Hardouin
SpeciesList	scientific name: Acinetobacter baumannii 1656-2; NCBI TaxID: 696749;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-09 02:09:34	ID requested
⏵ 1	2022-02-17 05:20:25	announced

Massier S, Robin B, M, é, groz M, Wright A, Harper M, Hayes B, Cosette P, Broutin I, Boyce JD, D, é E, Hardouin J, in Sessile Mode of Growth. Front Microbiol, 12():738780(2021) [pubmed]

submitter keyword: phosphorylation, Acinetobacter baumannii, biofilm, extracellular proteins, LC-MS/MS

Julie Hardouin
contact affiliation	Université de Rouen, Laboratoire PBS, France
contact email	julie.hardouin@univ-rouen.fr
lab head
Julie Hardouin
contact affiliation	Université de Rouen - laboratoire PBS
contact email	julie.hardouin@univ-rouen.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027208
     1. Label: PRIDE project
     2. Name: Phosphorylation of extracellular proteins in Acinetobacter baumannii in sessile mode of growth