PXD027207

PXD027207 is an original dataset announced via ProteomeXchange.

Title	Xenopus Hwa IP-MS in 1 cell stage embryos
Description	Spemann and Mangold Organizer (SMO) is of fundamental importance for the dorsal ventral body axis formation during vertebrate embryogenesis. Maternal Huluwa (Hwa) has been identified as the dorsal determinant that is both necessary and sufficient for the SMO formation. However, it remains unclear how Hwa is regulated. Here we report that the E3 ubiquitin ligase zinc and ring finger 3 (ZNRF3) is essential for restricting the spatial activity of Hwa, and therefore the proper SMO formation in Xenopus leavis. ZNRF3 interacts with and ubiquitinates Hwa thereby regulating its lysosomal trafficking and protein stability. Perturbation of ZNRF3 leads to the accumulation of Hwa and induction of ectopic axis in embryos. Ectopic expression of ZNRF3 promotes Hwa degradation and damps the axis-inducing activity of Hwa. Thus, our findings identify a substrate of ZNRF3, but also highlight the importance of the regulation of Hwa temporospatial activity in body axis formation in vertebrate embryos.
HostingRepository	PRIDE
AnnounceDate	2021-09-29
AnnouncementXML	Submission_2021-09-28_18:48:25.734.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhu xuechen
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-09 01:28:26	ID requested
⏵ 1	2021-09-28 18:48:26	announced

Dataset with its publication pending

submitter keyword: Xenopus Embryos Hwa IP-MS

Tao Qinghua
contact affiliation	School of Life Sciences, Tsinghua University, Beijing 100084, China
contact email	qhtaolab@tsinghua.edu.cn
lab head
zhu xuechen
contact affiliation	Westlake University
contact email	zhuxuechen@westlake.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD027207

PRIDE project URI

• PRIDE
  1. PXD027207
     1. Label: PRIDE project
     2. Name: Xenopus Hwa IP-MS in 1 cell stage embryos