PXD027206

PXD027206 is an original dataset announced via ProteomeXchange.

Title	RNA-mediated nucleosome depletion is required for elimination of transposon-derived DNA
Description	To study the role of chromatin remodeler during programmed genome reorganization in Paramecium tetraurelia. Paramecium ISWI1 was tagged with 3 FLAG and HA at its C-terminal. The recombinant plasmid was transformed into Paramecium and used for co-immunoprecipitation and Mass spectrometry studies to identify novel interacting partners of Paramecium ISWI1 protein that modulates the elimination of transposable elements.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:38:52.120.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Paramecium tetraurelia; NCBI TaxID: 5888;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-09 01:28:25	ID requested
1	2023-03-10 23:59:18	announced
⏵ 2	2023-11-14 08:38:53	announced	2023-11-14: Updated project metadata.

Singh A, Maurer-Alcal, á XX, Solberg T, H, ä, u, ß, ermann L, Gisler S, Ignarski M, Swart EC, Nowacki M, Chromatin remodeling is required for sRNA-guided DNA elimination in Paramecium. EMBO J, 41(22):e111839(2022) [pubmed]

submitter keyword: Paramecium tetraurelia, transposable elements, genome reorganization, nucleosome positioning, epigenetics Project,ISWI, chromatin

Mariusz Nowacki
contact affiliation	Institute of Cell Biology, University of Bern, Bern, Switzerland
contact email	mariusz.nowacki@izb.unibe.ch
lab head
Manfred Heller
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland
contact email	pmscf@dbmr.unibe.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD027206

PRIDE project URI

• PRIDE
  1. PXD027206
     1. Label: PRIDE project
     2. Name: RNA-mediated nucleosome depletion is required for elimination of transposon-derived DNA