To determine the acetylome of S. coelicolor, the wild-type S. coelicolor sample was prepared with three biological replicates and two technical replicates and analyzed. To quantify the acetylome and succinylome, three samples (wild-type, ∆SccobB1, and ∆SccobB2) with three biological replicates were prepared and analyzed. The cells were harvested, digested, and then subjected to affinity enrichment before LC-MS/MS analysis.