PXD027203

PXD027203 is an original dataset announced via ProteomeXchange.

Title	Evaluation of mass spectrometry MS/MS spectra for the presence of isopeptide crosslinked peptides
Description	Chronic low dose exposure to organophosphorus pesticides is associated with risk of neurodegenerative disease. The mechanism of neurotoxicity is unknown, though it is independent of acetylcholinesterase inhibition. Adducts on tyrosine, lysine, threonine, and serine can occur after exposure to organophosphorus pesticides, the most stable being adducts on tyrosine. Rabbit monoclonal 1C6 to diethoxyphosphate modified tyrosine (depY) was created by single B cell cloning. The amino acid sequence and binding constant (Kd 3.2 x 10-­‐8 M) were determined. Cultured human neuroblastoma SH-­‐SY5Y and mouse neuroblastoma N2a cells incubated with a sub-­‐cytotoxic dose of 10 µM chlorpyrifos oxon contained depY-­‐modified proteins detected by monoclonal 1C6 on Western blots. DepY-­labeled peptides from tryptic digests of cell lysates were imunopurified by binding to immobilized 1C6. Peptides released with 50% acetonitrile, 1% formic acid were analyzed by LC-­‐MS/MS on an Orbitrap Fusion Lumos mass spectrometer. Protein Prospector database searches identified 51 peptides modified on tyrosine by diethoxyphosphate in human SH-­‐SY5Y cell lysate and 73 diethoxyphosphate-­‐modified peptides in mouse N2a cell lysate. Adducts appeared most frequently on the cytoskeleton proteins tubulin, actin, and vimentin. It was concluded that rabbit monoclonal 1C6 can be useful for studies that aim to understand the mechanism of neurotoxicity resulting from low dose exposure t organophosphorus pesticides.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:03:24.166.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lawrence Schopfer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-09 01:28:10	ID requested
⏵ 1	2022-02-17 05:03:24	announced

Onder S, van Grol M, Fidder A, Xiao G, Noort D, Yerramalla U, Tacal O, Schopfer LM, Lockridge O, Rabbit Antidiethoxyphosphotyrosine Antibody, Made by Single B Cell Cloning, Detects Chlorpyrifos Oxon-Modified Proteins in Cultured Cells and Immunopurifies Modified Peptides for Mass Spectrometry. J Proteome Res, 20(10):4728-4745(2021) [pubmed]

submitter keyword: transglutaminase, isopeptide, crosslinks, MAP-rich tubulin, dansyl cadaverine, dansyl-aminohexyl QQIV, Protein Prospector, neurodegenerative disease

Oksana Lockridge
contact affiliation	Eppley Institute University of Nebraska Medical Center
contact email	olockrid@unmc.edu
lab head
Lawrence Schopfer
contact affiliation	University of Nebraska Medical Center
contact email	lmschopf@unmc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027203
     1. Label: PRIDE project
     2. Name: Evaluation of mass spectrometry MS/MS spectra for the presence of isopeptide crosslinked peptides