PXD027198 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Personalised phosphoproteomics extracts functional signalling |
Description | Protein phosphorylation dynamically integrates environmental and cellular information to control virtually all biological processes. Identifying functional phosphorylation amongst the thousands of phosphosites regulated by a perturbation at a global scale is a major challenge. Here, we introduce personalised phosphoproteomics, an experimental and computational framework to link signalling with biological function by exploiting human phenotypic variance. This is achieved by measuring individual subject phosphoproteome responses to interventions with corresponding phenotypes measured in parallel. We employed this method to investigate how exercise potentiates insulin sensitivity in human skeletal muscle. Our approach extracted signalling highly enriched in known functional sites, as well as many previously unknown sites on proteins intimately involved in glucose metabolism. This included a new co-operative relationship between mTOR and AMPK, for which we found a role in metabolic regulation. These results establish personalised phosphoproteomics as a general approach to investigate the signal transduction underlying complex biology. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-06 |
AnnouncementXML | Submission_2022-01-19_07:53:11.977.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sean Humphrey |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Exploris 480; Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-08 21:36:18 | ID requested | |
1 | 2021-12-06 09:37:22 | announced | |
⏵ 2 | 2022-01-19 07:53:12 | announced | 2022-01-19: Updated publication reference for PubMed record(s): 34857927. |
Publication List
Needham EJ, Hingst JR, Parker BL, Morrison KR, Yang G, Onslev J, Kristensen JM, H, ø, jlund K, Ling NXY, Oakhill JS, Richter EA, Kiens B, Petersen J, Pehm, ø, ller C, James DE, Wojtaszewski JFP, Humphrey SJ, Personalized phosphoproteomics identifies functional signaling. Nat Biotechnol, 40(4):576-584(2022) [pubmed] |
Keyword List
submitter keyword: phosphoproteomics, human, skeletal muscle, insulin, exercise |
Contact List
Sean J Humphrey |
contact affiliation | The University of Sydney, Australia |
contact email | sean.humphrey@sydney.edu.au |
lab head | |
Sean Humphrey |
contact affiliation | The University of Sydney |
contact email | sean.j.humphrey@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD027198 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027198
- Label: PRIDE project
- Name: Personalised phosphoproteomics extracts functional signalling