PXD027197

PXD027197 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic profiling of secreted extracellular vesicles from breast fibroadenoma and malignant lesion patients: a pilot study
Description	Exosomes are a subset of extracellular vesicles shuttling proteins, RNA, DNA and lipids crucial for cell–to–cell communication. Recent findings highlighted that exosomes, by virtue of their cargo, may also contribute to breast tumor growth and dissemination of metastasis. Indeed, these vesicles are gaining great interest as non-invasive cancer biomarkers. However, little is known on exosome biological and physical properties from breast malignant lesions and even less by non–malignant lesions such as breast fibroadenoma. The latter, being a non-malignant lesion, is clinically managed by conservative approaches. However, the molecular features of fibroadenoma are still largely unknown. In this pilot study, we attempt to purify and explore the proteomic profiling of breast benign and tumor exosomes from breast fibroadenoma, HER2+ and triple negative patient tumors as well from continuous cell lines by combining experimental and semi-quantitative approaches (BT-549, MCF10-A and MDA-MB-231). Interestingly, proteome-wide analyses showed 49 common proteins across breast fibroadenoma, HER2+, triple-negative malignant lesions and model cell exosomes. This is the first feasibility study evaluating physicochemical composition and proteome of extracellular vesicles from breast benign, malignant primary cells and immortalized cells. Our preliminary results may hold promises for freshly isolated primary cells protein identification with possible implications in breast cancer precision medicine.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:07.994.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nico Hüttmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-08 21:35:31	ID requested
1	2022-05-31 04:54:56	announced
⏵ 2	2023-11-14 08:53:08	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Breast cancer, exosome, early diagnosis,Fibroadenoma, extracellular vesicles, cell-to-cell signaling, precision medicine, disease monitoring

Gerolama Condorelli
contact affiliation	Department of Molecular Medicine and Medical Biotechnology, Federico II University of Naples, Naples, Italy
contact email	gecondor@unina.it
lab head
Nico Hüttmann
contact affiliation	John L. Holmes Mass Spectrometry Facility, Faculty of Science, University of Ottawa, Canada
contact email	nhutt069@uottawa.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD027197

PRIDE project URI

• PRIDE
  1. PXD027197
     1. Label: PRIDE project
     2. Name: Comparative proteomic profiling of secreted extracellular vesicles from breast fibroadenoma and malignant lesion patients: a pilot study