PXD027193

PXD027193 is an original dataset announced via ProteomeXchange.

Title	Characterization of waxy starches from CRISPR-Cas9 edited potato plants, the loss of GBSS1 influences the phosphate con-tent inside the starch granule
Description	The granule-bound starch synthase is responsible for amylose biosynthesis. This enzyme is ac-tive when physically associated with starch granules where it represents up to 95% of starch-bound proteins. gbss1 mutants of Solanum tuberosum, cv Desiree were recently generated by CRIPR/Cas9-driven gene edition. Here, we investigated the impact of the modulation of GBSS1 contents on the starch-bound proteome and the resulting amylopectin structure. The total protein content in knock-out mutants was decreased by 50 %, which indicate a compensation by the other proteins since GBSS1 represent 85 % of the total protein content. This compensation was mainly due to the increase of starch-bound SS2 (starch synthase 2) and GWD (glucan water dikinase). These changes were accompanied by an increase of the phosphate content. Taken to-gether, these data reveal that GBSS1 loss is compensated by the association of other starch met-abolic enzymes with starch granules, with a strong increase of GWD, leading to modified amy-lopectin structure.
HostingRepository	PRIDE
AnnounceDate	2026-01-26
AnnouncementXML	Submission_2026-01-26_07:29:13.819.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BRAY FABRICE
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: NEWT:4113;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-08 09:10:33	ID requested
⏵ 1	2026-01-26 07:29:14	announced

10.1016/j.ijbiomac.2026.150329;

Helle S, Sevestre F, Veillet F, Bray F, Flament S, Derbass W, Chauvin L, Rolando C, D'Hulst C, Chauvin JE, Szydlowski N, Loss of the granule-bound starch synthase protein reshapes the starch proteome and phosphorylation profile in potato tuber. Int J Biol Macromol, 343(Pt 2):150329(2026) [pubmed]

submitter keyword: Proteomics

Starch

GBSS1

waxy

GWD

phosphorylation

chain length distribution

metabolism

Nicolas Szydlowski
contact affiliation	Univ. Lille, CNRS, UMR8576 – UGSF – Unité de Glycobiologie Structurale et Fonctionnelle, F-59000 Lille, France Univ. Lille, CNRS, USR 3290 – MSAP – Miniaturisation pour la Synthèse l’Analyse et la Protéomique, F-59000 Lille, France
contact email	nicolas.szydlowski@univ-lille.fr
lab head
BRAY FABRICE
contact affiliation	MSAP USR 3290
contact email	fabrice.bray@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD027193

PRIDE project URI

• PRIDE
  1. PXD027193
     1. Label: PRIDE project
     2. Name: Characterization of waxy starches from CRISPR-Cas9 edited potato plants, the loss of GBSS1 influences the phosphate con-tent inside the starch granule