PXD027189

PXD027189 is an original dataset announced via ProteomeXchange.

Title	Sequence specific recognition of U1 snRNP stem-loop 4 by the ubiquitin-like domain of U2 snRNP protein SF3A1 and its RGG motif
Description	Cross-linking of isotope-labelled RNA coupled with mass spectrometry (CLIR-MS) was used to study the interaction between the ubiquitin-like domain (UBL) of the protein SF3A1, a component of the U2 snRNP, with stem-loop 4 RNA (SL4) from the U1 snRNP. The complex was cross-linked and analysed both in isolation, and in broader context with components of the U1 snRNP. Cross-linking was performed using irradiation under 254 nm light, relying on the inherent reactivity of ribonucleotides.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:38:47.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Sarnowski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-08 09:10:07	ID requested
⏵ 1	2022-02-22 04:38:48	announced

de Vries T, Martelly W, Campagne S, Sabath K, Sarnowski CP, Wong J, Leitner A, Jonas S, Sharma S, Allain FH, Sequence-specific RNA recognition by an RGG motif connects U1 and U2 snRNP for spliceosome assembly. Proc Natl Acad Sci U S A, 119(6):(2022) [pubmed]

submitter keyword: UV cross-linking

SF3A1

U1

U2

snRNP

splicing

spliceosome

structural proteomics

protein-RNA

RNA-protein

protein-RNA complex

RNA-protein complex

CLIR-MS

Alexander Leitner
contact affiliation	ETH Zurich, Institute of Molecular Systems Biology
contact email	leitner@imsb.biol.ethz.ch
lab head
Christopher Sarnowski
contact affiliation	Institute of Molecular Systems Biology, Department of Biology, ETH Zürich
contact email	sarnowski@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027189

PRIDE project URI

• PRIDE
  1. PXD027189
     1. Label: PRIDE project
     2. Name: Sequence specific recognition of U1 snRNP stem-loop 4 by the ubiquitin-like domain of U2 snRNP protein SF3A1 and its RGG motif