PXD027178 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Response of Endozoicomonas to coral host cues and thermal stress |
Description | Endozoicomonas are prevalent, abundant bacterial associates of marine animal hosts, including corals. Their role in holobiont health and functioning, however, remains poorly understood. To identify putative interactions within the coral holobiont, we characterized a novel Endozoicomonas isolate and assessed its transcriptomic and proteomic responses to tissue extracts of its native host, the Red Sea coral Acropora humilis, at control and elevated temperatures. We show that host cues stimulated differential expression of genes assumed to be involved in the modulation of the host immune response by Endozoicomonas, such as flagellar assembly genes, ankyrins, ephrins, and serpins. Proteome analysis revealed the upregulation of vitamin B1 and B6 biosynthetic as well as glycolytic processes by Endozoicomonas in response to host cues. We further demonstrate that the inoculation of A. humilis with its native Endozoicomonas strain resulted in enhanced holobiont health metrics, such as host tissue protein content and algal symbiont photosynthetic efficiency. Behavioral, physiological, and metabolic changes in Endozoicomonas may be key to the onset and function of mutualistic interactions within the coral holobiont, and our results suggest that the priming of Endozoicomonas to a symbiotic lifestyle may involve modulation of host immunity and the exchange of essential metabolites with other holobiont members. Consequently, Endozoicomonas presumably plays an important role in holobiont nutrient cycling and may therefore be implicated in its health, acclimatization, and ecological adaptation. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-12 |
AnnouncementXML | Submission_2022-08-12_08:32:06.184.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD027178 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Clinton Oakley |
SpeciesList | scientific name: Endozoicomonas; NCBI TaxID: 305899; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-07 21:47:58 | ID requested | |
⏵ 1 | 2022-08-12 08:32:07 | announced | |
Publication List
Pogoreutz C, Oakley CA, R, ä, decker N, C, á, rdenas A, Perna G, Xiang N, Peng L, Davy SK, Ngugi DK, Voolstra CR, Coral holobiont cues prime Endozoicomonas for a symbiotic lifestyle. ISME J, 16(8):1883-1895(2022) [pubmed] |
Keyword List
submitter keyword: Endozoicomonas, coral, symbiosis, coral reef, host-microbe interactions |
Contact List
Simon K Davy |
contact affiliation | School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand |
contact email | simon.davy@vuw.ac.nz |
lab head | |
Clinton Oakley |
contact affiliation | Victoria University of Wellington |
contact email | clint.oakley@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027178
- Label: PRIDE project
- Name: Response of Endozoicomonas to coral host cues and thermal stress