PXD027178

PXD027178 is an original dataset announced via ProteomeXchange.

Title	Response of Endozoicomonas to coral host cues and thermal stress
Description	Endozoicomonas are prevalent, abundant bacterial associates of marine animal hosts, including corals. Their role in holobiont health and functioning, however, remains poorly understood. To identify putative interactions within the coral holobiont, we characterized a novel Endozoicomonas isolate and assessed its transcriptomic and proteomic responses to tissue extracts of its native host, the Red Sea coral Acropora humilis, at control and elevated temperatures. We show that host cues stimulated differential expression of genes assumed to be involved in the modulation of the host immune response by Endozoicomonas, such as flagellar assembly genes, ankyrins, ephrins, and serpins. Proteome analysis revealed the upregulation of vitamin B1 and B6 biosynthetic as well as glycolytic processes by Endozoicomonas in response to host cues. We further demonstrate that the inoculation of A. humilis with its native Endozoicomonas strain resulted in enhanced holobiont health metrics, such as host tissue protein content and algal symbiont photosynthetic efficiency. Behavioral, physiological, and metabolic changes in Endozoicomonas may be key to the onset and function of mutualistic interactions within the coral holobiont, and our results suggest that the priming of Endozoicomonas to a symbiotic lifestyle may involve modulation of host immunity and the exchange of essential metabolites with other holobiont members. Consequently, Endozoicomonas presumably plays an important role in holobiont nutrient cycling and may therefore be implicated in its health, acclimatization, and ecological adaptation.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_08:32:06.184.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027178
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Clinton Oakley
SpeciesList	scientific name: Endozoicomonas; NCBI TaxID: 305899;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-07 21:47:58	ID requested
⏵ 1	2022-08-12 08:32:07	announced

Pogoreutz C, Oakley CA, R, ä, decker N, C, á, rdenas A, Perna G, Xiang N, Peng L, Davy SK, Ngugi DK, Voolstra CR, Coral holobiont cues prime Endozoicomonas for a symbiotic lifestyle. ISME J, 16(8):1883-1895(2022) [pubmed]

submitter keyword: Endozoicomonas, coral, symbiosis, coral reef, host-microbe interactions

Simon K Davy
contact affiliation	School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand
contact email	simon.davy@vuw.ac.nz
lab head
Clinton Oakley
contact affiliation	Victoria University of Wellington
contact email	clint.oakley@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027178
     1. Label: PRIDE project
     2. Name: Response of Endozoicomonas to coral host cues and thermal stress