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PXD027178

PXD027178 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleResponse of Endozoicomonas to coral host cues and thermal stress
DescriptionEndozoicomonas are prevalent, abundant bacterial associates of marine animal hosts, including corals. Their role in holobiont health and functioning, however, remains poorly understood. To identify putative interactions within the coral holobiont, we characterized a novel Endozoicomonas isolate and assessed its transcriptomic and proteomic responses to tissue extracts of its native host, the Red Sea coral Acropora humilis, at control and elevated temperatures. We show that host cues stimulated differential expression of genes assumed to be involved in the modulation of the host immune response by Endozoicomonas, such as flagellar assembly genes, ankyrins, ephrins, and serpins. Proteome analysis revealed the upregulation of vitamin B1 and B6 biosynthetic as well as glycolytic processes by Endozoicomonas in response to host cues. We further demonstrate that the inoculation of A. humilis with its native Endozoicomonas strain resulted in enhanced holobiont health metrics, such as host tissue protein content and algal symbiont photosynthetic efficiency. Behavioral, physiological, and metabolic changes in Endozoicomonas may be key to the onset and function of mutualistic interactions within the coral holobiont, and our results suggest that the priming of Endozoicomonas to a symbiotic lifestyle may involve modulation of host immunity and the exchange of essential metabolites with other holobiont members. Consequently, Endozoicomonas presumably plays an important role in holobiont nutrient cycling and may therefore be implicated in its health, acclimatization, and ecological adaptation.
HostingRepositoryPRIDE
AnnounceDate2022-08-12
AnnouncementXMLSubmission_2022-08-12_08:32:06.184.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD027178
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterClinton Oakley
SpeciesList scientific name: Endozoicomonas; NCBI TaxID: 305899;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-07 21:47:58ID requested
12022-08-12 08:32:07announced
Publication List
Pogoreutz C, Oakley CA, R, ä, decker N, C, á, rdenas A, Perna G, Xiang N, Peng L, Davy SK, Ngugi DK, Voolstra CR, Coral holobiont cues prime Endozoicomonas for a symbiotic lifestyle. ISME J, 16(8):1883-1895(2022) [pubmed]
Keyword List
submitter keyword: Endozoicomonas, coral, symbiosis, coral reef, host-microbe interactions
Contact List
Simon K Davy
contact affiliationSchool of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand
contact emailsimon.davy@vuw.ac.nz
lab head
Clinton Oakley
contact affiliationVictoria University of Wellington
contact emailclint.oakley@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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